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Hepatocyte Specific Antigen (Hepatocellular Marker) Antibody [MSVA-OCE5]

In Stock
Catalog Number Formulation Size Price
MSM5-20065-P0
Purified Ab with BSA and Azide
20ug
$259.00
MSM5-20065-P1
Purified Ab with BSA and Azide
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
N/A Antibody in Numerous Tissues (IHC)

N/A Antibody in Numerous Tissues (IHC)

N/A Mouse Monoclonal Antibody (MSVA-OCE5) tested on many normal and cancer tissues. The immunohistochemistry staining in these tissues aligns with the expression data in Human Protein Atlas.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1:100-1:200
IHC Protocol
Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Summary

Hepatocyte Specific Antigen, also called Hepatocyte Paraffin 1 or HepPar1, localizes to the mitochondria of hepatocytes. It is a sensitive marker for distinguishing hepatocellular carcinomas (HCC) from other metastatic carcinomas as well as cholangio-carcinomas. HCC's occur primarily in the stomach, but they are also found in many other organs. The Hepatocyte Specific Antigen may also be a useful marker for intestinal metaplasia. Reportedly, strong expression of the Hepatocyte Specific Antigen correlates with smaller tumor size and longer patient survival. Occasionally, Hepatocyte Specific Antigen is also found in gastric carcinomas as well as in a few other non-hepatic tumors.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cytoplasm
Gene Name
N/A
Positive Control
Liver: Virtually all hepatocytes should show an at least moderate granular cytoplasmic staining.
Immunogen
Extract of a formalin-fixed, rejected-allograft of a human liver
Alternate Names
N/A, N/A, Not Known

Database Links

Entrez Gene ID
Not Known
SwissProt
N/A

Additional Information

Clone
MSVA-OCE5
Mol. Weight of Antigen
Not Known
Purification
Protein G

Related Antibodies

View All N/A Antibodies

Functions

  • N/A

Key References

  • Ramos-Vara, J.A., et al. Histochem 2002; J. 34: 397-401.
  • Wennerberg AE et. al. Am J Pathol 1993;143:1050-4.
  • Fan, Z., et al. Mod. Pathol 2003; 16: 137-144, 2003.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

Ab purified from Bioreactor Concentrate by Protein G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
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