SDS-PAGE Analysis Purified DSG1 Mouse Monoclonal Antibody (18D4). Confirmation of Integrity and Purity of Antibody

SDS-PAGE Analysis Purified DSG1 Mouse Monoclonal Antibody (18D4). Confirmation of Integrity and Purity of Antibody

Printable Datasheet

Desmoglein-1 (DSG1) [18D4]

Clear

Summary

Recognizes a protein of ~150kDa, identified as Desmoglein-1 (DSG1). Desmoglein-1 is a member of the desmosomal cadherin family.Desmosomes are intercellular adhering junctions that represent cell surface attachment sites for intermediate filament. Desmocollins and desmogleins are the main desmosomal transmembrane proteins. Desmogleins consist of Dsg1, Dsg2, Dsg3, and Dsg4 isoforms.Within the desmosome, the extracellular domain of desmoglein is essential for calcium dependent heterophilic binding to the desmocollins, whereas the intracellular domain is essential for binding to the desmosomal plaque protein, plakoglobin. Desmoglein 1 is synthesized exclusively in the suprabasal layers. Intact and functionally active desmoglein 1 is essential to epidermal integrity.

Product Properties & Targets

Host
Mouse
Species Reactivity
Human
Research Areas
Autophagy
Isotype
IgG1
Cellular Localization
Cell junction, Cell membrane, Desmosome
Light Chain
kappa
Gene Name
DSG1
Positive Control
A431 or A-375 cells. Skin.
Immunogen
Human desmoglein-1 intracellular domain
Alternate Names
Desmoglein-1, Cadherin family member 4, Desmosomal glycoprotein 1, Pemphigus foliaceus antigen, Cadherin family member 4; CDHF4; Desmoglein-1; Desmosomal glycoprotein 1; DG1; DSG1; EPKHE; EPKHIA; Pemphigus foliaceus antigen; PPKS1; SPPK1

Database Links

Entrez Gene ID
1828
Unigene
2633
SwissProt
Q02413

Additional Information

Clone
18D4
Human Gene Symbol
DSG1
Chromosome Location
18q12.1
Mol. Weight of Antigen
150kDa (precursor); 160kDa (mature)

Functions

  • Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.

Known Applications & Suggested Dilutions

  • Flow Cytometry (1-2ug/million cells)
  • Immunofluorescence (1-2ug/ml)
  • Western Blot (1-2ug/ml) Optimal dilution for a specific application should be determined.

Key References

  • Wahl, J K III. Generation of monoclonal antibodies specific for desmoglein family members. Hybrid Hybridomics 2002, 21: 37
  • Wu, H., Stanley, J.R. and Cotsarelis, G. Desmoglein isotype expression in the hair follicle and its cysts correlates with type of keratinization and degree of differentiation. J Invest Dermatol 2003, 120: 1052-1057.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Reviews

There are no reviews yet.

Be the first to review “Desmoglein-1 (DSG1)”

Your email address will not be published. Required fields are marked *

Related

Recombinant mouse monoclonal antibodies and recombinant rabbit monoclonals to support your research

Shop Primary Antibodies

Company Info
Antibodies
View All
Hosts
View All
Isotype
View All
Resources
Newsletter Signup NeoBiotechnologies
Updates & Sales

$0.00
510-376-5603