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Desmoglein-1 (DSG1) Antibody [32-2B]

These antibodies perform exceptionally well in the intended applications and have undergone stringent testing and additional validation to ensure monospecificity.

The MAbMan recommends these antibodies for repeatable and reliable results.

Additional information

Catalog Number: 1828-MSM4
Reactivity:

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
1828-MSM4-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
1828-MSM4-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
1828-MSM4-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Desmoglein-1 (DSG1) Antibody

Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with Desmoglein-1 Mouse Monoclonal Antibody (32-2B).

Desmoglein-1 (DSG1) Antibody - Image 2

Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with Desmoglein-1 Mouse Monoclonal Antibody (32-2B).

Desmoglein-1 (DSG1) Antibody - Image 3

SDS-PAGE Analysis of Purified Desmoglein-1 Mouse Monoclonal Antibody (32-2B). Confirmation of Purity and Integrity of Antibody.

  • IHC
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Clone
32-2B
Isotype
IgG2a
Light Chain
Kappa
Cellular Localization
Cell junction, Cell membrane, Desmosome
Gene Name
DSG1
Positive Control
Bladder, skin or melanoma.
Immunogen
Bovine desmoglein (desmosomal glyco protein I) from nasal epidermis.
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.
Key References
  • Wu, H., et al. J Invest Dermatol 2003, 120: 1052-1057.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
1828
SwissProt
Q02413
Chromosome Location
18q12.1
Mol. Weight of Antigen
150kDa (precursor); 160kDa (mature)
Alternate Names
Desmoglein-1, Cadherin family member 4, Desmosomal glycoprotein 1, Pemphigus foliaceus antigen, Cadherin family member 4; CDHF4; Desmoglein-1; Desmosomal glycoprotein 1; DG1; DSG1; EPKHE; EPKHIA; Pemphigus foliaceus antigen; PPKS1; SPPK1

Summary

Recognizes a protein of ~150kDa, identified as Desmoglein-1 (DSG1). Desmoglein-1 is a member of the desmosomal cadherin family.Desmosomes are intercellular adhering junctions that represent cell surface attachment sites for intermediate filament. Desmocollins and desmogleins are the main desmosomal transmembrane proteins. Desmogleins consist of Dsg1, Dsg2, Dsg3, and Dsg4 isoforms.Within the desmosome, the extracellular domain of desmoglein is essential for calcium dependent heterophilic binding to the desmocollins, whereas the intracellular domain is essential for binding to the desmosomal plaque protein, plakoglobin. Desmoglein 1 is synthesized exclusively in the suprabasal layers. Intact and functionally active desmoglein 1 is essential to epidermal integrity.

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