Formalin-fixed, paraffin-embedded human Skin stained with Cytokeratin 15 Rabbit Recombinant Monoclonal Ab (KRT15/2103R).

Formalin-fixed, paraffin-embedded human Prostate stained with Cytokeratin 15 Rabbit Recombinant Monoclonal Ab (KRT15/2103R).

SDS-PAGE Analysis Purified Cytokeratin 15 Rabbit Recombinant Monoclonal Ab (KRT15/2103R). Confirmation of Purity and Integrity of Antibody.

Flow Cytometric Analysis of HeLa cells using KRT15 Rabbit Recombinant Monoclonal Ab (KRT15/2103R) followed by Goat anti-rabbit IgG-CF488 (Blue); Isotype Control (Red).

Printable Datasheet

Cytokeratin 15 (Esophageal Squamous Cell Carcinoma Marker) [KRT15/2103R]

Name: Cytokeratin 15 (Esophageal Squamous Cell Carcinoma Marker)
SKU: 3866-RBM3
Availability: InStock

Monoclonals Primary Antibodies Rabbit Monoclonals Recombinant Rabbit Monoclonals Recombinants

Summary

Keratin 15 is a type I keratin which is expressed only in basal keratinocytes in stratified epithelia and does not appear to have a natural type II expression partner. KRT15 is downregulated in activated keratinocytes. It is a specific marker of stem cells of the hair-follicle bulge and may be a useful marker for diagnosis between basal cell carcinoma (BCC) and trichoepithelioma. Trichoblastoma are benign neoplasms of follicular differentiation frequently found in nevus sebaceous. Many morphologic features are shared with nodular basal cell carcinoma, sometimes rendering a diagnosis difficult. Trichoblastoma and BCC show variable expression of Cytokeratin 15 and Cytokeratin 19, and absence of hair keratins.

Product Properties & Targets

Host

Rabbit

Species Reactivity

Cow, Human, Rat

Research Areas

Autophagy

Isotype

IgG

Gene Name

KRT15

Mol. Weight of Antigen

52kDa

Immunogen

Recombinant human full-length KRT15 protein

Database Links

Entrez Gene ID

3866

Unigene

654570

SwissProt

P19012

Additional Information

Clone

KRT15/2103R

Human Gene Symbol

KRT15

Chromosome Location

17q21.2

Known Applications & Suggested Dilutions

Flow Cytometry (1-2ug/million cells)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Key References

Yu-Hong Shen, et al. Asian Pac J Cancer Prev, 17 (9), 4217-4222.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified by Protein A Column. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.