SDS-PAGE Analysis Purified Cytochrome P450 1A1/1A2 Mouse Monoclonal Antibody (MC1). Confirmation of Purity and Integrity of Antibody.

SDS-PAGE Analysis Purified Cytochrome P450 1A1/1A2 Mouse Monoclonal Antibody (MC1). Confirmation of Purity and Integrity of Antibody.

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Cytochrome P450 1A1/1A2 [MC1]

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Summary

Cytochrome P450 oxidase (commonly abbreviated CYP) is a generic term for a large number of related, but distinct, oxidative enzymes important in vertebrate physiology. The cytochrome P450 mixed-function monooxygenase system is probably the most important element of Phase I metabolism in mammals. P450s are membrane-bound, either in the inner membrane of mitochondria or in the endoplasmic reticulum of cells where they metabolise thousands of endogenous and exogenous compounds. In the liver, these substrates include toxins, drugs, and other unneeded and potentially harmful molecules. Humans have 18 families of cytochrome P450 genes and 43 subfamilies; the CYP1 family is involved in drug metabolism and includes 3 subfamilies, 3 genes and 1 pseudogene.

Product Properties & Targets

Host
Mouse
Species Reactivity
Human, Mouse, Primate, Rat
Isotype
IgG1
Cellular Localization
Cytoplasm, Endoplasmic reticulum membrane, Microsome membrane, Mitochondrion inner membrane
Light Chain
kappa
Gene Name
CYP1A1
Positive Control
HeLa cells. Liver or Cardiac muscle.
Immunogen
3-methylcholanthrene induced rat cytochrome P450 protein.
Alternate Names
Cytochrome P450 1A1, Cytochrome P450 form 6, Cytochrome P450-C, Cytochrome P450-P1, Hydroperoxy icosatetraenoate dehydratase, Aryl hydrocarbon hydroxylase; CP11; CP12; CYP1A1; CYP1A2; CYPIA1; CYPIA2; Cytochrome P450 family 1 subfamily A polypeptide 1; Cytochrome P450 family 1 subfamily A polypeptide 2; P1 450; P3 450; P450 form 4; P450 form 6; P450 P1; P450 P3

Database Links

Entrez Gene ID
1543; 1544
Unigene
437060
SwissProt
P04798; P05177

Additional Information

Clone
MC1
Human Gene Symbol
CYP1A1 / CYP1A2
Chromosome Location
15q24.1
Mol. Weight of Antigen
58kDa

Functions

  • A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, steroid hormones and vitamins (PubMed:11555828, PubMed:14559847, PubMed:12865317, PubMed:15805301, PubMed:15041462, PubMed:18577768, PubMed:19965576, PubMed:20972997, PubMed:10681376). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH
  • hemoprotein reductase) (PubMed:11555828, PubMed:14559847, PubMed:12865317, PubMed:15805301, PubMed:15041462, PubMed:18577768, PubMed:19965576, PubMed:20972997, PubMed:10681376). Catalyzes the hydroxylation of carbon-hydrogen bonds. Exhibits high catalytic activity for the formation of hydroxyestrogens from estrone (E1) and 17beta-estradiol (E2), namely 2-hydroxy E1 and E2, as well as D-ring hydroxylated E1 and E2 at the C15-alpha and C16-alpha positions (PubMed:11555828, PubMed:14559847, PubMed:12865317, PubMed:15805301). Displays different regioselectivities for polyunsaturated fatty acids (PUFA) hydroxylation (PubMed:15041462, PubMed:18577768). Catalyzes the epoxidation of double bonds of certain PUFA (PubMed:15041462, PubMed:19965576, PubMed:20972997). Converts arachidonic acid toward epoxyeicosatrienoic acid (EET) regioisomers, 8,9-, 11,12-, and 14,15-EET, that function as lipid mediators in the vascular system (PubMed:20972997). Displays an absolute stereoselectivity in the epoxidation of eicosapentaenoic acid (EPA) producing the 17(R),18(S) enantiomer (PubMed:15041462). May play an important role in all-trans retinoic acid biosynthesis in extrahepatic tissues. Catalyzes two successive oxidative transformation of all-trans retinol to all-trans retinal and then to the active form all-trans retinoic acid (PubMed:10681376). May also participate in eicosanoids metabolism by converting hydroperoxide species into oxo metabolites (lipoxygenase-like reaction, NADPH-independent) (PubMed:21068195).

Known Applications & Suggested Dilutions

  • Immunofluorescence (1-2ug/ml)
  • Western Blot (1-2ug/ml)
  • Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)
  • Optimal dilution for a specific application should be determined.

Key References

  • Goshorn SG., et al. J Biol Chem 266:2134-2142 (1991).

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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