CTLA4 / CD152 (Negative Regulator of T-Cells) [L4P2F5.F10]

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Summary

CTLA4 (CD152) is a cell surface receptor that behaves as a negative regulator of the proliferation and the effector function of T cells. It contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases.The novel finding that CTLA-4 molecule is expressed and functional on human tumor cells opens up the possibility of antitumor therapeutic intervention based on targeting this molecule.

Product Properties & Targets

Host
Species Reactivity
Isotype
Cellular Localization
Cell membrane
Light Chain
Gene Name
Positive Control
Jurkat or Raji cells. Lymph node or tonsil.
Immunogen
A synthetic peptide from CTLA4 (exact sequence is proprietary)
Alternate Names
Cytotoxic T-lymphocyte protein 4, Cytotoxic T-lymphocyte-associated antigen 4, ALPS5; CD152; Celiac disease 3 (CELIAC3); Cytotoxic T-lymphocyte-associated antigen 4 (CTLA4); GRD4; GSE; ICOS; Insulin-dependent Diabetes Mellitus 12 (IDDM12)

Database Links

Entrez Gene ID
Unigene
SwissProt

Additional Information

Clone
L4P2F5.F10
Human Gene Symbol
CTLA4
Chromosome Location
2q33
Mol. Weight of Antigen
30-43kDa

Functions

  • Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28.

Known Applications & Suggested Dilutions

  • Flow Cytometry (1-2ug/million cells)
  • ,Immunofluorescence (1-2ug/ml)
  • ,Western Blot (1-2ug/ml)
  • ,Optimal dilution for a specific application should be determined.

Key References

  • Peach, R.J., et al. 1995. Both extracellular immunoglobulin-like domains of CD80 contain residues critical for binding T cell surface receptors CTLA-4 and CD28. J. Biol. Chem. 270: 21181-21187.
  • Fargeas, C.A., et al. 1995. Identification of residues in the V domain of CD80 (B7-1) implicated in functional interactions with CD28 and CTLA4. J. Exp. Med. 182: 667-675.

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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