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Recombinant CD99 / MIC2 (Ewing’s Sarcoma Marker) Antibody [MIC2/9939R]

In Stock
Catalog Number Formulation Size Price
4267-RBM26-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$249.00
4267-RBM26-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$539.00
4267-RBM26-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$539.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
CD99 Antibody in Western Blot (WB)

CD99 Antibody in Western Blot (WB)

Western blot analysis of K-562, MCF7, A549, A-431 & MDA-MB-231 cell lysates using CD99 Rabbit Recombinant Monoclonal Antibody (MIC2/9939R).

CD99 Antibody in Immunohistochemistry (IHC (P))

CD99 Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human pancreas stained with CD99 Rabbit Recombinant Monoclonal Antibody (MIC2/9939R). Inset: PBS instead of primary antibody; secondary only negative control.

CD99 Antibody in Immunohistochemistry (IHC (P))

CD99 Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human Ewing Sarcoma stained with CD99 Rabbit Recombinant Monoclonal Antibody (MIC2/9939R). HIER: Tris/EDTA, pH9.0, 45min. 2°: HRP-polymer, 30min. DAB, 5min.

CD99 Antibody in SDS-PAGE

CD99 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified CD99 Rabbit Recombinant Monoclonal Antibody (MIC2/9939R). Confirmation of Purity and Integrity of Antibody.

CD99 Antibody in Western Blot (WB)

CD99 Antibody in Western Blot (WB)

Western blot analysis of human brain, mouse brain, rat brain, hamster brain, guinea pig brain, rabbit brain, human kidney, mouse kidney, rat kidney, hamster kidney, guinea pig kidney, rabbit kidney, human lung, mouse lung, rat lung, hamster lung, guinea pig lung and rabbit lung tissue lysates using CD99 Rabbit Recombinant Monoclonal Antibody (MIC2/9939R).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Recognizes a sialoglycoprotein of 27-32kDa, identified as CD99, or MIC2 gene product, or E2 antigen. MIC2 gene is located in the pseudo-autosomal region of the human X and Y chromosome. MIC2 gene encodes two distinct proteins, which are produced by alternative splicing of the CD99 gene transcript and are identified as bands of 30 and 32kDa (p30/32). Although its function is not fully understood, CD99 is implicated in various cellular processes including homotypic aggregation of T cells, upregulation of T cell receptor and MHS molecules, apoptosis of immature thymocytes and leukocyte diapedesis. CD99 is expressed on the cell membrane of some lymphocytes, cortical thymocytes, and granulosa cells of the ovary. Most pancreatic islet cells, Sertoli cells of the testis, and some endothelial cells express this antigen. Mature granulocytes express very little or
no CD99. MIC2 is strongly expressed on Ewing’s sarcoma cells and primitive peripheral neuroectodermal tumors.

Product Properties & Targets

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Guinea Pig, Hamster, Human, Mouse, Rabbit, Rat
Research Areas
Immunology
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Membrane
Gene Name
CD99
Positive Control
K-562, A549, A-431, MCF7 or MDA-MB-231 cells. Human pancreas or Ewing's Sarcoma.
Immunogen
Recombinant fragment (around aa1-185) of human MIC2 protein (exact sequence is proprietary)
Alternate Names
CD99 antigen, 12E7, E2 antigen, Protein MIC2, T-cell surface glycoprotein E2, 12E7; E2 antigen; MIC 2X; MIC 2Y; MIC2; Protein MIC2; Surface antigen MIC2; T-cell surface glycoprotein E2

Database Links

Entrez Gene ID
4267
SwissProt
P14209

Additional Information

Clone
MIC2/9939R
Chromosome Location
Xp22.33
Mol. Weight of Antigen
27-32kDa

Related Antibodies

View All CD99 Antibodies

Functions

  • Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes (By similarity).

Key References

  • Ambros IM et. al. MIC2 is a specific marker for Ewing's sarcoma and peripheral primitive neuroectodermal tumors. Cancer 1991;67(1):1886-93.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified by Protein A Column. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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