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CD38 (ADP Ribosyl Cyclase I) Antibody [CD38/9332]

In Stock
Catalog Number Formulation Size Price
952-MSM22-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$249.00
952-MSM22-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$539.00
952-MSM22-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$539.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
CD38 Antibody in Western Blot (WB)

CD38 Antibody in Western Blot (WB)

Western blot analysis of Human Intestine, Human Spleen, Human Skeletal Muscle, Raji and Jurkat lysates using CD38 Mouse Monoclonal Antibody (CD38/9332).

CD38 Antibody in Immunohistochemistry (IHC (P))

CD38 Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human tonsil stained with CD38 Mouse Monoclonal Antibody (CD38/9332). HIER: Tris/EDTA, pH9.0, 45min. 2°: HRP-polymer, 30min. DAB, 5min.

CD38 Antibody in Immunohistochemistry (IHC (P))

CD38 Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human tonsil stained with CD38 Mouse Monoclonal Antibody (CD38/9332). Inset: PBS instead of primary antibody; secondary only negative control.

CD38 Antibody in SDS-PAGE

CD38 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified CD38 Mouse Monoclonal Antibody (CD38/9332). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

CD38 is a type II transmembrane glycoprotein that is present on early B- and T-cell lineages and activated B- and T-cells but is absent from most mature resting peripheral lymphocytes. CD38 is also found on thymocytes, pre-B cells, germinal center B-cells, mitogen-activated T-cells, monocytes and Ig-secreting plasma cells. CD38 is expressed on CD34+ cells. The CD34+CD38- population of hematopoietic stems cells define the most pluripotent cells (e.g. blast colony forming cells).

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
B Cell Markers, Hematopoietic Stem Cells
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Membrane
Gene Name
CD38
Positive Control
Raji cells. Human tonsil, spleen or skin, Human Intestine, Human Skeletal Muscle, Jurkat
Immunogen
Recombinant fragment (around aa1-200) of human CD38 protein (exact sequence is proprietary)
Alternate Names
ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1, 2'-phospho-ADP-ribosyl cyclase, 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase, 2'-phospho-cyclic-ADP-ribose transferase, ADP-ribosyl cyclase 1, Cyclic ADP-ribose hydrolase 1, T10, Acute Lymphoblastic Leukemia Cells Antigen; ADP Ribosyl Cyclase 1; cADP-ribose Hydrolase 1; CD38H; NAD(+) Nucleosidase; NIM-R5 Antigen; p45; T10

Database Links

Entrez Gene ID
952
SwissProt
P28907

Additional Information

Clone
CD38/9332
Chromosome Location
4p15
Mol. Weight of Antigen
~45kDa (Glycoprotein); 35kDa (protein core)

Related Antibodies

View All CD38 Antibodies

Functions

  • Synthesizes the second messengers cyclic ADP-ribose and nicotinate-adenine dinucleotide phosphate, the former a second messenger for glucose-induced insulin secretion. Also has cADPr hydrolase activity. Also moonlights as a receptor in cells of the immune system.

Key References

  • Deaglio S, et. al. J Immunol. 1998;160(1):395-402.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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