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Recombinant CD163 (Monocyte & Macrophage Marker) Antibody [M130/3708R]

In Stock
Catalog Number Formulation Size Price
9332-RBM8-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
9332-RBM8-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
9332-RBM8-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

CD163 is a type I membrane protein, and is a member of the hemoglobin scavenger receptor cystein-rich superfamily. The protein is involved in the clearance of hemoglobin-haptoglobin complexes and is considered to have anti-inflammatory functions. CD163 expression is restricted to the monocytic/macrophage lineage. It is expressed by all circulating monocytes and by a majority of tissue macrophages, such as splenic dendrocytes, alveolar macrophages and Kupffer cells of the liver. It is not present in macrophages in the mantle zone and some of the germinal center cells in lymph follicles, nor in Langerhans cells and interdigitating reticulum cells. In tumor tissues, CD163 is found in almost all cases of acute myeloid leukemia with monocytoid differentiation and in the majority of cases of histiocytic sarcoma, littoral cell angioma, Rosai-Dorfman disease, Langerhans cell histiocytosis and typical and atypical fibrous histiocytoma. It is also expressed in some cases of dermatofibrosarcoma protuberans. CD163 can be used to detect cells of monocytic and histiocyte lineage in neoplastic and reactive lesions. It has been shown to be more sensitive than CD68 for the detection of macrophages and monocytic cells. It covers a similar, but not identical, spectrum of cells as CD68.

Product Properties & Targets

Host
Rabbit
Applications
Species Reactivity
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cell membrane, Secreted
Gene Name
Positive Control
HPBL cells. Skin, Lung, placenta or histiocytoma tissue.
Immunogen
Synthetic peptide corresponding to CD163 residues within aa1056-1156 of CD163 was used as an immunogen
Alternate Names
Scavenger receptor cysteine-rich type 1 protein M130, Hemoglobin scavenger receptor, Hemoglobin scavenger receptor, MM130; Scavenger receptor cysteine rich type 1 protein M130

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
M130/3708R
Chromosome Location
12p13.3
Mol. Weight of Antigen
140kDa

Functions

  • Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells.
  • After shedding, the soluble form (sCD163) may play an anti-inflammatory role, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions.

Key References

  • Carpentieri DF et al. Mod Pathol. 2002 Oct;15(10):1080-6.
  • Goldstein NS et al. Am J Clin Pathol. 2002 Apr;117(4):541-5.

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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