Formalin-fixed, paraffin-embedded human Fetal Liver stained with AFP Mouse Monoclonal Antibody (MBS-12).

SDS-PAGE Analysis Purified AFP Mouse Monoclonal Antibody (MBS-12). Confirmation of Integrity and Purity of Antibody.

Printable Datasheet

AFP (Alpha Fetoprotein) (Hepatocellular/Germ Cell Tumor Marker) [MBS-12]

Name: AFP (Alpha Fetoprotein) (Hepatocellular/Germ Cell Tumor Marker)
SKU: 174-MSM4
Availability: InStock

Monoclonals Mouse Monoclonals Primary Antibodies

Summary

It recognizes an oncofetal glycoprotein with a single chain of 70kDa, which is identified as alpha fetoprotein (AFP). This MAb is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin. The yolk sac and the liver produce AFP during fetal life. AFP expression in adults is often associated with hepatoma or teratoma. However, hereditary persistence of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the AFP and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. AFP is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of AFP in amniotic fluid is used to measure renal loss of protein to screen for spinal bifida and anencephaly.

Product Properties & Targets

Host

Mouse

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry

Species Reactivity

Human

Research Areas

Cardiovascular

Isotype

IgG1

Cellular Localization

Secreted

Light Chain

Kappa

Gene Name

AFP

Positive Control

Hep-G2 cells. Fetal liver or hepatocellular carcinoma.

Immunogen

Human Alpha feto protein

Alternate Names

Alpha-fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin, Alpha fetoglobulin; FETA; HPAFP

Database Links

Entrez Gene ID

174

Unigene

518808

SwissProt

P02771

Additional Information

Clone

MBS-12

Human Gene Symbol

AFP

Chromosome Location

4q13.3

Mol. Weight of Antigen

70kDa

Functions

Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.

Known Applications & Suggested Dilutions

Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Key References

Tsung K., Milunsky A., Alpert E. Derivation and characterization of a monoclonal hybridoma antibody specific for human alpha-fetoprotein. J. Immunol. Methods 39: 363-368 (1980).
Michell B., Fiebach H., Karsten U., Goussev A.I., Yazova A.K., Knopp J. Monoclonal antibodies to different epitopes of human alpha-fetoprotein (AFP). Eur. J. Cancer Clin. Oncol. 19:1239-1246 (1983).
Yazova A.K., Goussev A.I., Poltorania V.S., Yakimenko E.F., Human alpha- fetoprotein epitopes as revealed by monoclonal antibodies. Immunol. Lett. 25: 325-330 (1990).

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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