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AFP (Alpha Fetoprotein) (Hepatocellular/Germ Cell Tumor Marker) Antibody [C3]

Additional information

Catalog Number: 174-MSM1
Reactivity:

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Gene:

Host:

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Catalog Number Formulation Size Price
174-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
174-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
174-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
AFP (Alpha Fetoprotein) (Hepatocellular/Germ Cell Tumor Marker) Antibody

Formalin-fixed, paraffin-embedded human Fetal Liver stained with AFP Mouse Monoclonal Antibody (C3).

Alpha-fetoprotein / AFP Antibody in SDS-PAGE

Alpha-fetoprotein / AFP Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified Alpha-fetoprotein / AFP Mouse Monoclonal Antibody (C3). Confirmation of Purity and Integrity of Antibody.

AFP (Alpha Fetoprotein) (Hepatocellular/Germ Cell Tumor Marker) Antibody - Image 3

SDS-PAGE Analysis of Purified AFP Mouse Monoclonal Antibody (C3). Confirmation of Integrity and Purity of Antibody.

  • IHC
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Dog, Human, Monkey, Pig
Clone
C3
Isotype
IgG2a
Light Chain
Kappa
Cellular Localization
Secreted
Gene Name
AFP
Positive Control
Hep-G2 cells. Fetal liver or hepatocellular carcinoma.
Immunogen
Alpha feto protein (AFP) Purified from serum of a hepatoma patient
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Reactivity:

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Other Information

Functions
  • Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
Key References
  • Tsung K., Milunsky A., Alpert E. Derivation and characterization of a monoclonal hybridoma antibody specific for human alpha-fetoprotein. J. Immunol. Methods 39: 363-368 (1980).
  • Michell B., Fiebach H., Karsten U., Goussev A.I., Yazova A.K., Knopp J. Monoclonal antibodies to different epitopes of human alpha-fetoprotein (AFP). Eur. J. Cancer Clin. Oncol. 19:1239-1246 (1983).
  • Yazova A.K., Goussev A.I., Poltorania V.S., Yakimenko E.F., Human alpha- fetoprotein epitopes as revealed by monoclonal antibodies. Immunol. Lett. 25: 325-330 (1990).
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
174
SwissProt
P02771
Chromosome Location
4q13.3
Mol. Weight of Antigen
70kDa
Alternate Names
Alpha-fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin, Alpha fetoglobulin; FETA; HPAFP

Summary

It recognizes an oncofetal glycoprotein with a single chain of 70kDa, which is identified as alpha-fetoprotein (AFP) (ISOBM TD-2 workshop). This MAb is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin. AFP is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors.

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