2 Union Square, Union City, CA 94587
contact@NeoBiotechnologies.com
510-376-5603

Home >> Antibodies >> TNF-alpha (Tumor Necrosis Factor alpha)

TNF-alpha (Tumor Necrosis Factor alpha)

Mouse Monoclonal Antibody [Clone J2D10]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
img

SDS-PAGE Analysis Purified TNF-alpha Mouse Monoclonal Antibody (J2D10). Confirmation of Purity and Integrity of Antibody.

Product Details

Synonyms

APC1, Cachectin, Differentiation inducing factor (DIF), Macrophage cytotoxic factor (MCF), Necrosin, TNF alpha, TNF Macrophage Derived, TNF Monocyte Derived, TNF Superfamily Member 2, TNFA, TNFSF2, Tumor necrosis factor ligand superfamily member 2, Tumor Necrosis Factor Precursor

Positive Control

HeLa, HL-60 or A431 cells. Macrophages in lymph node or Tonsil.

Known Applications & Suggested Dilutions

Flow Cyt, IF         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Inhibits Tumor Growth (Order Ab without BSA/Azide)
Neutralizes rTNF- Mediated Cytotoxicity (Order Ab without BSA/Azide)
Protects Mice Against Toxicity of rhTNF- (Order Ab without BSA/Azide)Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human TNF-alpha

Cellular Localization

Cytoplasmic and extracellular (secreted)

Species Reactivity

Human. Does not react with Mouse.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

17kDa

Specificity & Comments

This antibody neutralises HurTNFamediated cytotoxicity of L929 cells and inhibits tumour growth in mice. It protects mice against toxicity of HurTNFa. Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17kDa band following SDS PAGE under non-reducing conditions. TNF alpha is closely related to the 25kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production.

Related Products



200ug/ml of Ab purified by Protein L. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Key References

  1. McLaughlin PJ; Elwood NJ; Russell SM; Andrew SM; McKenzie IF. Properties of monoclonal antibodies to human tumor necrosis factor alpha (TNF alpha). Anticancer Research, 1992, 12(4):1243-6.
  2. McLaughlin PJ; Elwood NJ; Ramadi LT, Pica MR, McKenzie IF. Improvement in sensitivity of enzyme-linked immunosorbent assay for tumor necrosis factor. Immunol Cell Biol, 1990, 68:51-5.

Bioinformatics

  •  Entrez Gene ID: 7124
  •  Gene Symbol: TNF
  •  SwissProt: P01375
  •  Chromosome Location: 6p21.33
  •  Unigene: 241570
NBT00994