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Home >> Antibodies >> TNF-alpha (Tumor Necrosis Factor alpha)

TNF-alpha (Tumor Necrosis Factor alpha)

Mouse Monoclonal Antibody [Clone J1D9]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00

Product Details

Synonyms

APC1, Cachectin, Differentiation inducing factor (DIF), Macrophage cytotoxic factor (MCF), Necrosin, TNF alpha, TNF Macrophage Derived, TNF Monocyte Derived, TNF Superfamily Member 2, TNFA, TNFSF2, Tumor necrosis factor ligand superfamily member 2, Tumor Necrosis Factor Precursor

Positive Control

HeLa, HL-60 or A431 cells. Macrophages in lymph node or Tonsil.

Known Applications & Suggested Dilutions

Flow Cyt, IF         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Inhibits Tumor Growth (Order Ab without BSA/Azide)
Neutralizes rTNF- Mediated Cytotoxicity (Order Ab without BSA/Azide)
Protects Mice Against Toxicity of rhTNF- (Order Ab without BSA/Azide)Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human TNF-alpha

Cellular Localization

Cytoplasmic and extracellular (secreted)

Species Reactivity

Human. Does not react with Mouse.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

17kDa

Specificity & Comments

This antibody neutralizes TNF alpha biological activities. It prevents TNF alpha induced apoptosis in Jurkat cells. It also neutralizes HurTNFamediated cytotoxicity of L929 cells and inhibits tumor growth in mice. It protects mice against toxicity of HurTNFa. Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17kDa band following SDS PAGE under non-reducing conditions. TNF alpha is closely related to the 25kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. McLaughlin PJ; Elwood NJ; Russell SM; Andrew SM; McKenzie IF. Properties of monoclonal antibodies to human tumor necrosis factor alpha (TNF alpha). Anticancer Research, 1992, 12(4):1243-6.
  2. Kalli K R, Kaufmann S H, et al. Molecular Pharmacology 64(6): 1434-1443 (2003).
  3. M. Paparakis, et al, Cytokine and Growth Factor Reviews 7: 223-229 (1996).
  4. P. Vassalli, Annu Rev Immunol 10: 411-452 (1992).
  5. R. Pijnenborg, et al, Placenta 19(4): 231-239 (1998).
  6. A. Eigler, et al, Immunology Today 18: 487-492 (1997).

Bioinformatics

  •  Entrez Gene ID: 7124
  •  Gene Symbol: TNF
  •  SwissProt: P01375
  •  Chromosome Location: 6p21.33
  •  Unigene: 241570
NBT00993