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Home >> Antibodies >> OLIG2 (Marker of Glial Brain Tumors)

OLIG2 (Marker of Glial Brain Tumors)

Mouse Monoclonal Antibody [Clone OLIG2/2400]

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Catalog No Formulation Size Price (USD)  
10215-MSM1-P0 Purified Ab with BSA and Azide at 200ug/ml 20 ug 199.00
10215-MSM1-P1 Purified Ab with BSA and Azide at 200ug/ml 100 ug 429.00
10215-MSM1-P1ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 100 ug 429.00
Human Entrez Gene ID 10215
Human SwissProt Q13516
Human Unigene 176977
Human Gene Symbol OLIG2
Human Chromosome Location 21q22.11
Synonyms Basic helix loop helix protein class B1 (bHLHB1); basic helix-loop-helix protein 19 (bHLHe19); OLIG2; Oligodendrocyte lineage transcription factor 2; Oligodendrocyte specific bHLH transcription factor 2; Oligodendrocyte transcription factor 2; Protein kinase C-binding protein 2 (PRKCBP2); RACK17
Immunogen Recombinant fragment of human OLIG2 protein (around aa 1-141) (exact sequence is proprietary)
Host / Ig Isotype Mouse / IgG1, kappa
Mol. Weight of Antigen 30-40kDa
Cellular Localization Nuclear
Species Reactivity Human. Others not known.

Specificity & Comments

Olig2, a basic helix-loop-helix transcription factor, is involved in oligodendroglial specification. Olig2 expression has been reported in most glial tumors, such as oligodendrogliomas and astrocytomas. Although more than half of glioblastomas are positive for Olig2, expression is very weak in terms of both percentage of labeled cells and intensity. No Olig2 expression has been found in the non-glial tumors including neuro-epithelial tumors, ependymomas, sub-ependymomas, medulloblastomas, and non-neuroepithelial tumors, such as CNS lymphomas, meningiomas, schwannomas, atypical teratoid / rhabdoid tumor, and haemangioblastomas. Compared to the strong staining seen in glioma samples, a weak expression is observed in non-tumoral brain tissue (gliosis).

Known Applications & Suggested Dilutions

Immunohistology (Formalin-fixed) (1-2ug/ml for 30 min at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Positive Control

THP-1 cells. Cerebellum or Astrocytoma.

Supplied As

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Storage and Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Key References

  1. Mokhtari K, et al. Olig2 expression, GFAP, p53 and 1p loss analysis contribute to glioma subclassification. Neuropathol Appl Neurobiol. 2005; 31:62-9.
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