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Recombinant OLIG2 (Marker of Glial Brain Tumors) Antibody [OLIG2/6695R]

Additional information

Catalog Number: 10215-RBM2
Reactivity:

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
10215-RBM2-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
10215-RBM2-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
10215-RBM2-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant OLIG2 (Marker of Glial Brain Tumors) Antibody

Formalin-fixed, paraffin-embedded human cerebrum stained with OLIG2 Recombinant Rabbit Monoclonal Antibody (OLIG2/6695R).

Recombinant OLIG2 (Marker of Glial Brain Tumors) Antibody - Image 2

Formalin-fixed, paraffin-embedded human oligodendroglioma stained with OLIG2 Recombinant Rabbit Monoclonal Antibody (OLIG2/6695R).

Recombinant OLIG2 (Marker of Glial Brain Tumors) Antibody - Image 3

Formalin-fixed, paraffin-embedded human cerebellum stained with OLIG2 Recombinant Rabbit Monoclonal Antibody (OLIG2/6695R).

Recombinant OLIG2 (Marker of Glial Brain Tumors) Antibody - Image 4

Formalin-fixed, paraffin-embedded human glioma stained withOLIG2 Recombinant Rabbit Monoclonal Antibody (OLIG2/6695R).

Recombinant OLIG2 (Marker of Glial Brain Tumors) Antibody - Image 5

IHC analysis of formalin-fixed, paraffin-embedded human brain. Stained with OLIG2/6695R at 2ug/ml in PBS for 30min RT. Inset: PBS instead of primary; secondary antibody negative control.

OLIG2 Antibody in SDS-PAGE

OLIG2 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified Oligodendrocyte transcription factor 2 Recombinant Rabbit Monoclonal Antibody (OLIG2/6695R). Confirmation of Purity and Integrity of Antibody.

  • IHC
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Product Properties

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry
Species Reactivity
Human
Clone
OLIG2/6695R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Cytoplasm, Nucleus
Gene Name
OLIG2
Positive Control
THP-1 cells. Human brain or astrocytoma.
Immunogen
Recombinant fragment (around aa200-300) of human OLIG2 protein (exact sequence is proprietary)
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Functions together with ZNF488 to promote oligodendrocyte differentiation. Cooperates with OLIG1 to establish the pMN domain of the embryonic neural tube. Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development.
Key References
  • Shou JW et al. Front Cell Neurosci 13:395 (2019). https://doi.org/10.3389/fncel.2019.00395
  • Cho KHT et al. Sci Rep 10:6904 (2020). https://doi.org/10.1038/s41598-020-59902-7
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
10215
SwissProt
Q13516
Chromosome Location
21q22.11
Mol. Weight of Antigen
32kDa
Alternate Names
Oligodendrocyte transcription factor 2, Class B basic helix-loop-helix protein 1, Class E basic helix-loop-helix protein 19, Protein kinase C-binding protein 2, Protein kinase C-binding protein RACK17, Basic helix loop helix protein class B1 (bHLHB1); basic helix-loop-helix protein 19 (bHLHe19); OLIG2; Oligodendrocyte lineage transcription factor 2; Oligodendrocyte specific bHLH transcription factor 2; Oligodendrocyte transcription factor 2; Protein kinase C-binding protein 2 (PRKCBP2); RACK17

Summary

Olig2, a basic helix loop helix transcription factor, is involved in oligo-dendroglial specification. Olig2 expression has been reported in most glial tumors, such as oligodendrogliomas and astrocytomas. Although more than half of glioblastomas are positive for Olig2, expression is very weak in terms of both percentage of labeled cells and intensity. No Olig2 expression has been found in the non-glial tumors including neuroepithelial tumors, ependymomas, sub-ependymomas, medulloblastomas, and non-neuroepithelial tumors, such as CNS lymphomas, meningiomas, schwannomas, atypical teratoid/rhabdoid tumor, and haemangioblastomas. Compared to the strong staining seen in glioma samples, a weak expression is observed in non-tumoral brain tissue.

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