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Home >> Antibodies >> NKX2.2 (Neuroendocrine & Ewing's Sarcoma Marker)

NKX2.2 (Neuroendocrine & Ewing's Sarcoma Marker)

Mouse Monoclonal Antibody [Clone NX2/294]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
img

Formalin-fixed, paraffin-embedded human Ewing's Sarcoma stained with NKX2.2 Mouse Monoclonal Antibody (NX2/294).

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Formalin-fixed, paraffin-embedded human Pancreas stained with NKX2.2 Mouse Monoclonal Antibody (NX2/294).

img

Formalin-fixed, paraffin-embedded Rat Pancreas stained with NKX2.2 Mouse Monoclonal Antibody (NX2/294).

img

SDS-PAGE Analysis Purified NKX2.2 Mouse Monoclonal Antibody (NX2/294).

Product Details

Synonyms

Homeobox protein NK-2 homolog B, NK2 transcription factor like protein B, NK2 transcription factor related locus 2, NKX22, Nkx2b, tinman

Positive Control

Pancreas or Ewing's sarcoma.

Known Applications & Suggested Dilutions

Flow Cyt, IF, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Human full-length recombinant NKX2.2 protein

Cellular Localization

Nuclear

Species Reactivity

Human. Mouse. Rat. Chicken.

Host / Ig Isotype

Mouse / IgG2b, kappa

Mol. Weight of Antigen

40-50kDa

Specificity & Comments

Expression of NKX2.2 has been found in neuroendocrine tumors of the gut, making it a potential marker for the study of gastrointestinal neuroendocrine tumors. More recently, NKX2.2 protein was identified as a target of EWS-FLI-1, the fusion protein specific to Ewing sarcoma, and was shown to be differentially upregulated in Ewing sarcoma on the basis of array-based gene expression analysis. It acts as a valuable marker for Ewing sarcoma, with a sensitivity of 93% and a specificity of 89%, and aids in the differential diagnosis of small round cell tumors.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Wang, Y.C. et al. (2009) Endocr Relat Cancer 16, 267-79.
  2. Yoshida A et. al. Am J Surg Pathol. 2012;36(7):993-9.

Bioinformatics

  •  Entrez Gene ID: 4821
  •  Gene Symbol: NKX2-2
  •  SwissProt: O95096
  •  Chromosome Location: 20p11.22
  •  Unigene: 516922
NBT00294