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Home >> Antibodies >> N-Cadherin / Cadherin-2 / CD325 (NCAD)

N-Cadherin / Cadherin-2 / CD325 (NCAD)

Mouse Monoclonal Antibody [Clone 8C11]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
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Formalin-fixed, paraffin-embedded mouse heart stained with N-Cadherin Mouse Monoclonal Antibody (8C11).

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SDS-PAGE Analysis Purified N-Cadherin Mouse Monoclonal Antibody (8C11). Confirmation of Integrity and Purity of Antibody.

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Western blot analysis of Heart tissue lysate using N-Cadherin Mouse Monoclonal Antibody (8C11).

Product Details

Synonyms

Cadherin-2 N cadherin neuronal; Cadherin-2 type 1; Cadherin-2; Calcium dependent adhesion protein neuronal; CD325; CDH2; CDHN; CDw325; N-Cadherin; NCAD

Positive Control

HeLa or HUVEC cells. Human heart, pancreas or cerebral cortex (IHC).

Known Applications & Suggested Dilutions

Flow Cyt, IF, WB, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blotting (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tis with1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human N-cadherin extracellular domain

Cellular Localization

Cell surface

Species Reactivity

Human and Mouse. Others not known.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

130-140kDa

Specificity & Comments

Recognizes a protein of ~140kDa, identified as N-Cadherin (NCAD), also known as CD325. NCAD is a member of the Cadherin superfamily, and consists of five extracellular repeats, a transmembrane domain and a cytoplasmic domain. CD325 deficient mice die at day 10 of gestation and embryos display major heart defects and malformed neural tubes and somites. Consistent with this, CD325 has been implicated in several aspects of cardiac development including the precardiac mesoderm, establishment of left-right symmetry and cardiac looping morphogenesis. Furthermore, CD325 is normally involved in inducing cell cycle arrest and its expression is frequently deregulated in cancer cells.Studies have linked N-cadherin to cancer metastasis by showing the aggressive tumor cells had preferentially turned on N-cadherin as opposed to E- or P-cadherin.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Wahl, JK III et al.N-cadherin-catenin complexes form prior to cleavage of the proregion and transport to the plasma membrane. J Biol Chem. 2003, 278(19):17269-76

Bioinformatics

  •  Entrez Gene ID: 1000
  •  Gene Symbol: CDH2
  •  SwissProt: P19022
  •  Chromosome Location: 18q11.2
  •  Unigene: 464829