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N-Cadherin / Cadherin-2 / CD325 (NCAD) Antibody [CDH2/9488]

Additional information

Catalog Number: 1000-MSM16
Reactivity:

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
1000-MSM16-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
1000-MSM16-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
1000-MSM16-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
N-Cadherin Antibody in Western Blot (WB)

N-Cadherin Antibody in Western Blot (WB)

Western Blot Analysis of A549 lysate using N-Cadherin Mouse Monoclonal Antibody (CDH2/9488).

CDH2 Antibody in SDS-PAGE

CDH2 Antibody in SDS-PAGE

SDS-PAGE Analysis Purified CDH2 Mouse Monoclonal Antibody (CDH2/9488). Confirmation of Purity and Integrity of Antibody.

N-Cadherin Antibody in Immunohistochemistry (IHC (P))

N-Cadherin Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human heart stained with N-Cadherin Mouse Monoclonal Antibody (CDH2/9488) Inset: PBS instead of primary antibody; secondary only negative control.

N-Cadherin Antibody in Western Blot (WB)

N-Cadherin Antibody in Western Blot (WB)

Western Blot Analysis of human heart tissue lysate using N-Cadherin Mouse Monoclonal Antibody (CDH2/9488).

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
CDH2/9488
Isotype
IgG2b
Light Chain
Kappa
Cellular Localization
Cell junction, Cell membrane, Cell surface, Sarcolemma
Gene Name
CDH2
Positive Control
HeLa or HUVEC cells. Human heart, pancreas or cerebral cortex.
Immunogen
Recombinant fragment (around aa 438-524) of human CDH2 protein (exact sequence is proprietary)
Purification
Protein A
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Recombinant N-Cadherin / Cadherin-2 / CD325 (NCAD) Antibody

Additional information

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Gene:

Host:

Reactivity:

, , ,

Formalin-fixed, paraffin-embedded human heart stained with N-Cadherin Recombinant Mouse Monoclonal Antibody (rCDH2/1426).
2
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  • WB

Recombinant N-Cadherin / Cadherin-2 / CD325 (NCAD) Antibody

Additional information

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Gene:

Host:

Reactivity:

VIEW ALL ANTIBODIES

Other Information

Functions
  • Calcium-dependent cell adhesion protein; preferentially mediates homotypic cell-cell adhesion by dimerization with a CDH2 chain from another cell. Cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. Plays a role in cell-to-cell junction formation between pancreatic beta cells and neural crest stem (NCS) cells, promoting the formation of processes by NCS cells (By similarity). CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density.
Key References
  • Wahl, JK III; Kim, YJ; Cullen, JM; Johnson, KR; Wheelock, MJ.N-cadherin-catenin complexes form prior to cleavage of the proregion and transport to the plasma membrane. J Biol Chem. 2003, 278(19):17269-76
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
1000
SwissProt
P19022
Chromosome Location
18q11.2
Mol. Weight of Antigen
130-140kDa
Alternate Names
Cadherin-2, CDw325, Neural cadherin, Cadherin-2 N cadherin neuronal; Cadherin-2 type 1; Cadherin-2; Calcium dependent adhesion protein neuronal; CD325; CDH2; CDHN; CDw325; N-Cadherin; NCAD

Summary

Recognizes a protein of ~140kDa, identified as N-Cadherin (NCAD), also known as CD325. NCAD is a member of the Cadherin superfamily, and consists of five extracellular repeats, a transmembrane domain and a cytoplasmic domain. CD325 deficient mice die at day 10 of gestation and embryos display major heart defects and malformed neural tubes and somites. Consistent with this, CD325 has been implicated in several aspects of cardiac development including the precardiac mesoderm, establishment of left-right symmetry and cardiac looping morphogenesis. Furthermore, CD325 is normally involved in inducing cell cycle arrest and its expression is frequently deregulated in cancer cells. Studies have linked N-cadherin to cancer metastasis by showing the aggressive tumor cells had preferentially turned-on N-cadherin as opposed to E- or P-cadherin.

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