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MUC1 / CA15-3 / EMA / CD227 (Epithelial Marker)

Recombinant Mouse Monoclonal Antibody [Clone rMUC1/960]

Printable

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

199.00

459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00

459.00

Printable

Bioinformatics

Formalin-fixed, paraffin-embedded human breast carcinoma stained with MUC1 Mouse Recombinant Monoclonal Antibody (rMUC1/960).

SDS-PAGE Analysis Purified MUC1 Mouse Recombinant Monoclonal Antibody (rMUC1/960). Confirmation of Purity and Integrity of Antibody.

Western Blot Analysis of human MCF-7 cell lysate using MUC1 Mouse Recombinant Monoclonal Antibody (rMUC1/960).

Formalin-fixed, paraffin-embedded human breast carcinoma stained with MUC1 Mouse Recombinant Monoclonal Antibody (rMUC1/960).

SDS-PAGE Analysis Purified MUC1 Mouse Recombinant Monoclonal Antibody (rMUC1/960). Confirmation of Purity and Integrity of Antibody.

Western Blot Analysis of human MCF-7 cell lysate using MUC1 Mouse Recombinant Monoclonal Antibody (rMUC1/960).

Product Details

Synonyms

Breast carcinoma-associated antigen DF3, CA15-3, Carcinoma-associated mucin Episialin, H23AG, KL-6, MAM6; MUC-1/SEC, MUC-1/X, MUC1-alpha, MUC1-beta, MUC1-CT, MUC1-NT, MUC1/ZD, Mucin-1 subunit beta, Peanut-reactive urinary mucin, PEM, PEMT, Polymorphic epithelial mucin, PUM, Tumor-associated epithelial membrane antigen

Positive Control

MCF-7 or MDA-231 cells. Human breast, colon, ovarian or endometrial carcinoma.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human MUC1 protein

Cellular Localization

Cytoplasm. Cell surface.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2b, kappa

Mol. Weight of Antigen

265-400kDa

Specificity & Comments

This MAb reacts with MUC1, a large transmembrane glycoprotein expressed on the ductal surface of normal glandular epithelia. The dominant epitope of this MAb involves both amino acids as well as sugar moieties. Neuraminidase treatment destroys the antigen. It is a very good tracer agent in CA15.3 assays. The extracellular domain of MUC1 largely consists of a highly conserved, O-glycosylated 20 amino acids tandem repeat which can occur 30-100 times per molecule depending on the length of the allele involved. In the vast majority of human carcinomas this protein is up-regulated and poorly glycosylated and appears on the cell surface in a non-polarized fashion.