Formalin-fixed, paraffin-embedded human breast carcinoma stained with MUC1 Mouse Recombinant Monoclonal Antibody (rMUC1/960).
SDS-PAGE Analysis Purified MUC1 Mouse Recombinant Monoclonal Antibody (rMUC1/960). Confirmation of Purity and Integrity of Antibody.
Western Blot Analysis of human MCF-7 cell lysate using MUC1 Mouse Recombinant Monoclonal Antibody (rMUC1/960).
Known Applications & Suggested Dilutions
Host / Ig Isotype
Mol. Weight of Antigen
Specificity & Comments
This MAb reacts with MUC1, a large transmembrane glycoprotein expressed on the ductal surface of normal glandular epithelia. The dominant epitope of this MAb involves both amino acids as well as sugar moieties. Neuraminidase treatment destroys the antigen. It is a very good tracer agent in CA15.3 assays. The extracellular domain of MUC1 largely consists of a highly conserved, O-glycosylated 20 amino acids tandem repeat which can occur 30-100 times per molecule depending on the length of the allele involved. In the vast majority of human carcinomas this protein is up-regulated and poorly glycosylated and appears on the cell surface in a non-polarized fashion.