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Home >> Antibodies >> Melanoma Marker (MART-1 + Tyrosinase + gp100)

Melanoma Marker (MART-1 + Tyrosinase + gp100)

Mouse Monoclonal Antibody [Clone DT101+ BC199 + T311 + HMB45]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
img

Formalin-fixed, paraffin-embedded human Melanoma stained with Melanoma Marker Mouse Monoclonal (DT101+ BC199 + T311 + HMB45).

Product Details

Synonyms

Melanoma antigen recognized by T-cells 1 (MART-1), MLAN-A; PMEL17

Positive Control

SK-MEL-13 and SK-MEL-19 melanoma cell lines. Human melanoma tissues.

Known Applications & Suggested Dilutions

IHC-P         More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant hMART-1 protein (DT101 & BC199); Extract of pigmented melanoma metastases from lymph nodes (HMB45); Recombinant tyrosinase protein (T311)

Cellular Localization

Cytoplasmic

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2b, kappa + Mouse / IgG1, kappa + Mouse / IgG2a, kappa

Mol. Weight of Antigen

20-22kDa (doublet) (MART-1): 90-100kDa (gp100); 70-80kDa (Tyrosinase)

Specificity & Comments

This antibody cocktail recognizes three melanoma-specific proteins, which include MART-1, Tyrosinase and gp100. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Function of gp100 is not known but it is reported to be a useful marker for melanocytes and melanomas. This cocktail of three markers is designed for extremely sensitive labeling of formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.

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200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Kawakami Y, et. al. Journal of Immunological Methods, 1997, 202(1):13-25.
  2. Chen Y-T, et. al. Proc Natl Acad Sci, USA, 1996, 93:5915-19.
  3. Gown AM, et. al. American Journal of Pathology, 1986, 123(2):195-203.

Bioinformatics

  •  Entrez Gene ID: 2315, 6490
NBT00704