Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with HSP27 Monoclonal Antibody (G3.1).
Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with HSP27 Monoclonal Antibody (G3.1).
Western Blot Analysis of HeLa cell lysate using HSP27 Monoclonal Antibody (G3.1).
SDS-PAGE Analysis of Purified HSP27 MAb (G3.1). Confirmation of Integrity and Purity of Antibody
Immunofluorescence Analysis of PFA-fixed MCF-7 cells labeling HSP27 with HSP27 MAb (G3.1) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red)
Flow Cytometric Analysis of PFA-fixed MCF-7 cells using HSP27 MAb (G3.1) followed by Goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).
Known Applications & Suggested Dilutions
Host / Ig Isotype
Mol. Weight of Antigen
Specificity & Comments
MAb G3.1 reacts specifically with heat shock protein HSP27 in human and monkey tissues and cell lines such as MCF-7. HSP27, also referred to as the Estrogen-Regulated 24K protein and HSP28, is one of several small heat shock proteins produced by all organisms studied. HSP27 synthesis is induced by elevated temperature, as well as by estrogen in hormone responsive cells. Interestingly, human HSP27 also shares greater than 50% homology with low molecular weight Drosophila HSPs and mammalian alpha-crystalline lens protein. Because of the estrogen responsive nature of HSP27, this protein has been studied extensively in human estrogen responsive tissues such as cervix, endometrium and breast tissue. Therefore HSP27 may be useful in classifying various hormone sensitive tumors.