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Home >> Antibodies >> HSP27 (Heat Shock Protein 27)

HSP27 (Heat Shock Protein 27)

Mouse Monoclonal Antibody [Clone G3.1]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
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Formalin-fixed, paraffin-embeddedhuman prostate carcinoma stained with HSP27 Mouse Monoclonal Antibody (G3.1).

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Formalin-fixed, paraffin-embeddedhuman breast carcinoma stained with HSP27 Mouse Monoclonal Antibody (G3.1).

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Western Blot Analysis of HeLa cell lysate using HSP27 Mouse Monoclonal Antibody (G3.1).

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SDS-PAGE Analysis Purified HSP27 Mouse Monoclonal Antibody (G3.1). Confirmation of Integrity and Purity of Antibody

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Immunofluorescence Analysis of PFA-fixed MCF-7 cells labeling HSP27 with HSP27 Mouse Monoclonal Antibody (G3.1) followed by goat anti-mouse IgG-CF488 (green). Nuclear counterstain is RedDot.

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Flow Cytometric Analysis of PFA-fixed MCF-7 cells using HSP27 Mouse Monoclonal Antibody (G3.1) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Product Details

Synonyms

Heat shock 27kDa protein, Estrogen-regulated 24kDa protein, Heat shock 25kDa protein 1, Heat shock 28kDa protein 1, Heat shock protein beta-1, HMN2B, Hsp25, Hsp28, HspB1, Stress-responsive protein 27 (SRP27)

Positive Control

MCF-7 or HeLa cells. ~50% of breast carcinomas are positive for hsp27, especially those that are positive for ER and/or PgR.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Partially purified hsp27 (earlier called 24K) protein from breast cancer MCF-7 cells.

Cellular Localization

Predominantly cytoplasmic with some nuclear

Species Reactivity

Human, Chimpanzee, Monkey, Sheep, Rat, Mouse and Chicken.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

27kDa

Specificity & Comments

MAb G3.1 reacts specifically with heat shock protein HSP27 in human and monkey tissues and cell lines such as MCF-7. HSP27, also referred to as the Estrogen-Regulated 24K protein and HSP28, is one of several small heat shock proteins produced by all organisms studied. HSP27 synthesis is induced by elevated temperature, as well as by estrogen in hormone responsive cells. Interestingly, human HSP27 also shares greater than 50% homology with low molecular weight Drosophila HSPs and mammalian alpha-crystalline lens protein. Because of the estrogen responsive nature of HSP27, this protein has been studied extensively in human estrogen responsive tissues such as cervix, endometrium and breast tissue. Therefore, HSP27 may be useful in classifying various hormone sensitive tumors.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Key References

  1. Edwards DP et. al.BiochemBiophys Research Commun, 93:804-812, 1980.
  2. Ciocca DR et. al. Breast Cancer Research and Treatment, 20:33-42, 1991.

Bioinformatics

  •  Entrez Gene ID: 3315
  •  Gene Symbol: HSPB1
  •  SwissProt: P04792
  •  Chromosome Location: 7q11.23
  •  Unigene: 520973