Home >> Antibodies >> Cytokeratin 7 (Glandular and Transitional Epithelial Marker)

Cytokeratin 7 (Glandular and Transitional Epithelial Marker)

Mouse Monoclonal Antibody [Clone SPM270]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Key References

Bioinformatics

Formalin-fixed, paraffin-embedded human Lung SCC stained with Cytokeratin 7 Monoclonal Antibody (SPM270).

Western Blot Analysis of human HeLa cell lysate using Cytokeratin 7 Monoclonal Antibody (SPM270).

Immunofluorescence Analysis of HeLa cells labeling Cytokeratin 7 with KRT7 Mouse Monoclonal Antibody (SPM270) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).

Flow Cytometric Analysis of trypsinised MeOH-fixed HeLa cells using KRT7 Mouse Monoclonal Antibody (SPM270) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Formalin-fixed, paraffin-embedded human Lung SCC stained with Cytokeratin 7 Monoclonal Antibody (SPM270).

Western Blot Analysis of human HeLa cell lysate using Cytokeratin 7 Monoclonal Antibody (SPM270).

Immunofluorescence Analysis of HeLa cells labeling Cytokeratin 7 with KRT7 Mouse Monoclonal Antibody (SPM270) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).

Flow Cytometric Analysis of trypsinised MeOH-fixed HeLa cells using KRT7 Mouse Monoclonal Antibody (SPM270) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Product Details

Synonyms

CK-7, K2C7, Keratin 55K Type II Cytoskeletal, Keratin Simple Epithelial Type 1 K7, Keratin Type II Cytoskeletal 7, Krt2-7, KRT7, Sarcolectin, SCL, Type II Mesothelial Keratin K7, Type-II Keratin Kb7

Positive Control

HeLa cells. Carcinoma of ovary, lung, cervix or breast.

Known Applications & Suggested Dilutions

Western Blot, Flow Cyt, IF, IHC-P More Details

Western Blot (1-2ug/ml)
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

OTN 11, ovarian carcinoma cell line

Cellular Localization

Cytoplasmic

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

55kDa

Specificity & Comments

It recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This MAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This MAb is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).