Home >> Antibodies >> Cytokeratin 5/6 (Basal, Myoepithelial & Mesothelial Cell Marker)

Cytokeratin 5/6 (Basal, Myoepithelial & Mesothelial Cell Marker)

Mouse Monoclonal Antibody [Clone KRT5.6/4866]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Key References

Bioinformatics

Formalin-fixed, paraffin-embedded human squamous cell carcinoma stained with Cytokeratin 5/6 Mouse Monoclonal Antibody (KRT5.6/4866).

Formalin-fixed, paraffin-embedded human prostate stained with Cytokeratin 5/6 Mouse Monoclonal Antibody (KRT5.6/4866).

Formalin-fixed, paraffin-embedded human squamous cell carcinoma stained with Cytokeratin 5/6 Mouse Monoclonal Antibody (KRT5.6/4866).

Formalin-fixed, paraffin-embedded human prostate stained with Cytokeratin 5/6 Mouse Monoclonal Antibody (KRT5.6/4866).

Product Details

Synonyms

CK5; Keratin-5; KRT5; Cytoskeletal 6A; KRT6A

Positive Control

Human prostate or skin tissue.

Known Applications & Suggested Dilutions

IHC-P More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human KRT5 protein

Cellular Localization

Cytoplasmic

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa & Mouse / IgG2a, kappa

Mol. Weight of Antigen

58kDa (KRT5); 56kDa (KRT6A)

Specificity & Comments

It recognizes polypeptides of 58kDa and 56kDa, identified as cytokeratin 5 and 6 respectively. It shows no reaction with keratin 1, 8, or 19. It stains the basal cells of squamous and glandular epithelia, part of stratum spinosum of squamous epithelium, myoepithelium, and mesothelium. It does not stain adenous epithelium. Therefore, this antibody can be used to distinguish squamous carcinoma from adenocarcinoma, and to differentiate epitheliod mesothelioma from non-epitheliod mesothelioma and adenocarcinoma. It is also a useful antibody in the identification of mesothelioma and lung squamous cell carcinoma. It does not react with pulmonary adenocarcinoma.