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Home >> Antibodies >> Cyclin A2 (S- & G2-phase Cyclin)

Cyclin A2 (S- & G2-phase Cyclin)

Mouse Monoclonal Antibody [Clone E67]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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SDS-PAGE Analysis Purified Cyclin A2 Mouse Monoclonal Antibody (E67). Confirmation of Integrity and Purity of Antibody.

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Formalin-fixed, paraffin-embedded human Endometrium stained with Cyclin A2 Mouse Monoclonal Antibody (E67).

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Western Blot Analysis of HeLa cell lysate using Cyclin A2 Mouse Monoclonal Antibody (E67).

Product Details

Synonyms

CCN1, CCNA, CCNA2, CCNA2_HUMAN, Cyclin A2

Positive Control

K-562 and HeLa cells. Human tonsil or lymph node.

Known Applications & Suggested Dilutions

Flow Cyt, IF, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Full length bovine Cyclin A protein

Cellular Localization

Predominantly nuclear

Species Reactivity

Human. Mouse. Cow. African Green Monkey. Mink (Mustela).

Host / Ig Isotype

Mouse / IgG2a, kappa

Mol. Weight of Antigen

54kDa

Specificity & Comments

It recognizes a protein of 54kDa, which is identified as cyclin A. Its epitope is located amino acids 144-148 of human Cyclin A2. Cyclins are regulatory subunits of the cyclin-dependent kinases (cdk's) and they control transition at different specific phases of the cell cycle. The temporal expression of cyclins is tightly regulated and subsequently plays a critical role in controlling the enzymatic activity of cdk's. These cyclin/cdk complexes are essential for passage through specific stages in the cell cycle. In mammalian somatic cells, cyclin A is required for S-phase and passage through G2-phase. The D and E type cyclins regulate the passage of G1, while cyclin B is a critical regulator of mitosis. Mutation or disruption of normal cyclin A expression causes cells to arrest in G2-phase.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Adamczewski JP; Gannon JV; Hunt T. Simian virus 40 large T antigen associates with cyclin A and p33cdk2. Journal of Virology, 1993, 67(11):6551-7.

Bioinformatics

  •  Entrez Gene ID: 890
  •  Gene Symbol: CCNA2
  •  SwissProt: P20248
  •  Chromosome Location: 4q27
  •  Unigene: 58974
NBT00673