Cyclin A2 (S- & G2-phase Cyclin) Antibody [E67]

Additional information

Catalog Number: 890-MSM1
Reactivity:

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Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

In Stock
Catalog Number Formulation Size Price
890-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
890-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
890-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
  • Flow
  • IF
  • IHC

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Flow Cytometry (Flow) 1-2ug/million cells Flow Cytometry Protocol
Immunofluorescence (IF) 1-3ug/ml
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Product Properties

Antibody Type
Host
Mouse
Clone
E67
Isotype
IgG2a
Light Chain
Kappa
Cellular Localization
Cytoplasm, Nucleus
Gene Name
Positive Control
K-562 and HeLa cells. Human tonsil or lymph node.
Immunogen
Full length bovine Cyclin A protein
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

Related Antibodies

Other Information

Functions
Key References
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
890
SwissProt
Chromosome Location
4q27
Mol. Weight of Antigen
54kDa
Alternate Names
Cyclin-A2, Cyclin A, CCN1, CCNA, CCNA2, CCNA2_HUMAN, Cyclin A2

Summary

It recognizes a protein of 54kDa, which is identified as cyclin A. Its epitope is located amino acids 144-148 of human Cyclin A2. Cyclins are regulatory subunits of the cyclin-dependent kinases (cdk’s) and they control transition at different specific phases of the cell cycle. The temporal expression of cyclins is tightly regulated and subsequently plays a critical role in controlling the enzymatic activity of cdk’s. These cyclin/cdk complexes are essential for passage through specific stages in the cell cycle. In mammalian somatic cells, cyclin A is required for S-phase and passage through G2-phase. The D and E type cyclins regulate the passage of G1, while cyclin B is a critical regulator of mitosis. Mutation or disruption of normal cyclin A expression causes cells to arrest in G2-phase.

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