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Home >> Antibodies >> Collagen VII

Collagen VII

Mouse Monoclonal Antibody [Clone LH7.2]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
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Formalin-fixed, paraffin-embedded human Skin stained with Collagen VII Mouse Monoclonal Antibody (LH7.2).

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Formalin-fixed, paraffin-embedded human Skin stained with Collagen VII Mouse Monoclonal Antibody (LH7.2).

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SDS-PAGE Analysis Purified Collagen VII Mouse Monoclonal Antibody (LH7.2). Confirmation of Purity and Integrity of Antibody.

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Immunofluorescence Analysis of PFA-fixed HeLa cells labeling Collagen VII using Collagen VII Mouse Monoclonal Antibody (LH7.2) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Redot.

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Flow Cytometric Analysis of PFA-fixed HeLa cells using Collagen VII Mouse Monoclonal Antibody (LH7.2) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red)

Product Details

Synonyms

Alpha 1 type VII collagen; Collagen 7; Collagen alpha 1(VII) chain; Collagen type VII alpha 1; Collagen VII alpha 1 polypeptide Col7a1; EBD1; EBDCT; EBR1; Long-chain (LC) collagen

Positive Control

HeLa, cells. Human lymphocytes. Human lymph node or tonsil.

Known Applications & Suggested Dilutions

ELISA, Western Blot, IF, IHC-P         More Details

ELISA (For coating, order antibody without BSA)
Western Blot (1-2ug/ml)Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human Collagen VII protein

Cellular Localization

Secreted. Extracellular matrix. Basement Membrane.

Species Reactivity

Human. Sheep. Goat. Horse. Guinea Pig. Cow.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

290kDa

Specificity & Comments

Recognizes an epitope located on collagenase digested Type VII collagen (150kDa), i.e. the non-helical carboxyl terminal region of the dimer. Binding is localized at the inferior border of the lamina densa as demonstrated by immunoelectron microscopy. Type VII is also found in the retina, where its function is unknown. It interacts with Laminin 5 and Fibronectin. Collagen VII is a stratified squamous epithelial basement membrane protein that forms anchoring fibrils which may contribute to epithelial basement membrane organization and adherence by interacting with extracellular matrix (ECM) proteins such as type IV collagen.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Ponsioen TL et al. Mol Vis 14:652-60 (2008).

Bioinformatics

  •  Entrez Gene ID: 1294
  •  Gene Symbol: COL7A1
  •  SwissProt: Q02388
  •  Chromosome Location: 3p21.31
  •  Unigene: 476218
NBT02315