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Home >> Antibodies >> CD99 / MIC2 (Ewing's Sarcoma Marker)

CD99 / MIC2 (Ewing's Sarcoma Marker)

Mouse Monoclonal Antibody [Clone MIC2/877]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
img

Formalin-fixed, paraffin-embedded human Ewing's sarcoma stained with CD99 Monoclonal Antibody (MIC2/877).

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Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with CD99 Monoclonal Antibody (MIC2/877).

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Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with CD99 Monoclonal Antibody (MIC2/877).

Product Details

Synonyms

12E7; E2 antigen; MIC 2X; MIC 2Y; MIC2; Protein MIC2; Surface antigen MIC2; T-cell surface glycoprotein E2

Positive Control

MOLT-4 cells. Pancreas or Ewing's sarcoma.

Known Applications & Suggested Dilutions

Flow Cyt, IF, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at Room Temp)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human MIC2 protein

Cellular Localization

Cell surface

Species Reactivity

Human. Does not react with Rat.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

27-32kDa

Specificity & Comments

Recognizes a sialoglycoprotein of 27-32kDa, identified as CD99, or MIC2 gene product, or E2 antigen. MIC2 gene is located in the pseudo-autosomal region of the human X and Y chromosome. MIC2 gene encodes two distinct proteins, which are produced by alternative splicing of the CD99 gene transcript and are identified as bands of 30 and 32kDa (p30/32).Although its function is not fully understood, CD99 is implicated in various cellular processes including homotypic aggregation of T cells, upregulation of T cell receptor and MHS molecules, apoptosis of immature thymocytes and leukocyte diapedesis.CD99 is expressed on the cell membrane of some lymphocytes, cortical thymocytes, and granulosa cells of the ovary. Most pancreatic islet cells, Sertoli cells of the testis, and some endothelial cells express this antigen. Mature granulocytes express very little or no CD99. MIC2 is strongly expressed on Ewing's sarcoma cells and primitive peripheral neuroectodermal tumors.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Ambros IM et. al. MIC2 is a specific marker for Ewing's sarcoma and peripheral primitive neuroectodermal tumors. Cancer 1991;67(1):1886-93.

Bioinformatics

  •  Entrez Gene ID: 4267
  •  Gene Symbol: CD99
  •  SwissProt: P14209
  •  Chromosome Location: Xp22.33
  •  Unigene: 653349
NBT00877