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Home >> Antibodies >> CD43 (T-Cell Marker)

CD43 (T-Cell Marker)

Mouse Monoclonal Antibody [Clone Bra7G]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
img

Formalin-fixed, paraffin-embedded human Spleen stained with CD43 Mouse Monoclonal Antibody (Bra7G).

img

Immunofluorescence Analysis of K562 cells labeling CD43 with CD43 Monoclonal Antibody (Bra7G) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is NucSpot’.

Product Details

Synonyms

Galactoglycoprotein, GALGP, GPL115, Leukocyte sialoglycoprotein, Leukosialin, LSN, Sialophorin, SPN

Positive Control

K562 cells, Paracortex in a tonsil or a reactive lymph node.

Known Applications & Suggested Dilutions

Flow Cyt, IF, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(For staining of formalin-fixed tissues, digest sections with trypsin at 1mg/ml PBS, 15 min at room temp)
Optimal dilution for a specific application should be determined.

Immunogen

Immature pluripotent human leukemia K562 cells

Cellular Localization

Cell surface

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgM, kappa

Mol. Weight of Antigen

95, 115, or 135kDa

Specificity & Comments

It recognizes a cell surface glycoprotein of 95/115/135kDa (depending upon the extent of glycosylation), identified as CD43 (Workshop V). Epitope of MAb Bra7G is clearly different from that of MAb DF-T1, called b as opposed to a for DF-T1. 70-90% of T-cell lymphomas and from 22-37% of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by PEG precipitation. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Chorvath B, et. al. Neoplasma, 1992, 39:325-9.
  2. Turzova M, et. al. Neoplasma, 1993, 40:9-13.

Bioinformatics

  •  Entrez Gene ID: 6693
  •  Gene Symbol: SPN
  •  SwissProt: P16150
  •  Chromosome Location: 16p11.2
  •  Unigene: 632188
NBT00302