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CD43 (T-Cell Marker) Antibody [DF-T1]

Additional information

Catalog Number: 6693-MSM1
Reactivity:

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
6693-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
6693-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
6693-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
CD43 (T-Cell Marker) Antibody

Formalin-fixed, paraffin-embeddedhuman spleen stained with CD43 Mouse Monoclonal Antibody (DF-T1).

CD43 (T-Cell Marker) Antibody - Image 2

Flow Cytometric Analysis of PFA fixed K562 cells using CD43 Mouse Monoclonal Antibody (DF-T1) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

CD43 (T-Cell Marker) Antibody - Image 3

Immunofluorescence Analysis of K562 cells labeling CD43 with CD43 Mouse Monoclonal Antibody (DF-T1) followed by goat anti-mouse IgG-CF488 (green). Nuclear counterstain is NucSpot Live 650.

CD43 (T-Cell Marker) Antibody - Image 4

Western blot analysis of K562 cell lysate using CD43 Mouse Monoclonal Antibody (DF-T1).

CD43 (T-Cell Marker) Antibody - Image 5

SDS-PAGE Analysis of Purified CD43 Mouse Monoclonal Antibody (DF-T1) Confirmation of Integrity and Purity of Antibody.

  • Flow
  • IF
  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Flow Cytometry (Flow) 1-2ug/million cells Flow Cytometry Protocol
Immunofluorescence (IF) 1-3ug/ml
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
DF-T1
Isotype
IgG1
Light Chain
Kappa
Cellular Localization
Cell projection, Membrane, Microvillus, Nucleus, PML body, Uropodium
Gene Name
SPN
Positive Control
PBMCs or K562cells (FC). K562 cell lysate (WB).Paracortex in a tonsil or a reactive lymph node (IHC).
Immunogen
Myeloblastic KG1 cells
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Predominant cell surface sialoprotein of leukocytes which regulates multiple T-cell functions, including T-cell activation, proliferation, differentiation, trafficking and migration. Positively regulates T-cell trafficking to lymph-nodes via its association with ERM proteins (EZR, RDX and MSN) (By similarity). Negatively regulates Th2 cell differentiation and predisposes the differentiation of T-cells towards a Th1 lineage commitment. Promotes the expression of IFN-gamma by T-cells during T-cell receptor (TCR) activation of naive cells and induces the expression of IFN-gamma by CD4(+) T-cells and to a lesser extent by CD8(+) T-cells (PubMed:18036228). Plays a role in preparing T-cells for cytokine sensing and differentiation into effector cells by inducing the expression of cytokine receptors IFNGR and IL4R, promoting IFNGR and IL4R signaling and by mediating the clustering of IFNGR with TCR (PubMed:24328034). Acts as a major E-selectin ligand responsible for Th17 cell rolling on activated vasculature and recruitment during inflammation. Mediates Th17 cells, but not Th1 cells, adhesion to E-selectin. Acts as a T-cell counter-receptor for SIGLEC1 (By similarity).
  • Protects cells from apoptotic signals, promoting cell survival.
Key References
  • Stross WP, et. al.Journal of Clinical Pathology, 1989, 42(9):953-61.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
6693
SwissProt
P16150
Chromosome Location
16p11.2
Mol. Weight of Antigen
115|95|or 135kDa
Alternate Names
Leukosialin, GPL115, Galactoglycoprotein, Leukocyte sialoglycoprotein, Sialophorin, Galactoglycoprotein, GALGP, GPL115, Leukocyte sialoglycoprotein, Leukosialin, LSN, Sialophorin, SPN

Summary

It recognizes a cell surface glycoprotein of 95/115/135kDa (depending upon the extent of glycosylation), identified as CD43 [Workshop IV]. Epitope of MAb Bra7G is clearly different from that of MAb DF-T1, called b as opposed to a for DF-T1. 70-90% of T-cell lymphomas and from 22-37% of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.

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