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Home >> Antibodies >> CD4, Mouse (T-Cell Marker)

CD4, Mouse (T-Cell Marker)

Rat Monoclonal Antibody [Clone GK1.5]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00

Product Details

Synonyms

CD4 (L3T4); CD4; CD4+ Lymphocyte deficiency, included; Leu3; Ly-4; Lymphocyte antigen CD4; p55; T cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4

Positive Control

Spleen, Thymus or Lymph Node.

Known Applications & Suggested Dilutions

ELISA, Neut. Studies, Flow Cyt, IF         More Details

ELISA (For coating, order Ab without BSA & Azide)
Neutralization Studies (Order Ab without BSA & Azide)
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Immunogen

Mouse CTL clone V4

Cellular Localization

Cell Surface

Species Reactivity

Mouse. Does not react with Human.

Host / Ig Isotype

Rat / IgG2b, kappa

Mol. Weight of Antigen

55kDa

Specificity & Comments

CD4 is a member of the Ig superfamily, primarily expressed on most thymocytes, a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a coreceptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosine kinase, lck. CD4 is expressed by the majority of thymocytes, most helper T cells, a subset of NK-T cells and weakly by dendritic cells and macrophages. CD4 plays an important role in the development of T cells and is required for mature T cells to function optimally.This antibody blocks helper T cell responses to MHC class II antigens, including cytolysis, proliferation, allogeneic B cell help, and release of lymphokines.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Frei K, et al. 1997. J. Exp. Med. 185:2177. (IHC)
  2. Wu L, et al. 1991. J. Exp. Med. 174:1617. (Costim)
  3. Godfrey DI, et al. 1994. J. Immunol. 152:4783. (Block)
  4. Gavett SH, et al. 1994. Am. J. Respir. Cell. Mol. Biol. 10:587. (Deplete)
  5. Felix NJ, et al. 2007. Nat. Immunol. 8:388. (Block)
  6. Schuyler M, et al. 1994. Am. J. Respir. Crit. Care Med. 149:1286. (Deplete)
  7. Zheng B, et al. 1996. J. Exp. Med. 184:1083. (IHC)

Bioinformatics

  •  Entrez Gene ID: 12504
  •  SwissProt: P06332
  •  Chromosome Location: 6 F2; 6 59.17 cM (Mouse)
  •  Unigene: 2209
NBT02009