IHC analysis of formalin-fixed, paraffin-embedded human tonsil using rBLCAM/6749 at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. Inset: PBS instead of primary antibody, secondary negative control.
Formalin-fixed, paraffin-embedded human tonsil stained with CD22 Recombinant Mouse Monoclonal Antibody (rBLCAM/6749).
SDS-PAGE Analysis Purified CD22 Recombinant Mouse Monoclonal Antibody (rBLCAM/6749). Confirmation of Purity and Integrity of Antibody.
Known Applications & Suggested Dilutions
Host / Ig Isotype
Mol. Weight of Antigen
Specificity & Comments
Recognizes a protein of 130-140kDa, identified as CD22 (also known as BL-CAM). CD22 expression is restricted to normal and neoplastic B cells and is absent from other haemopoietic cell types. In B-cell ontogeny, CD22 is first expressed in the cytoplasm of pro-B and pre-B cells, and on the surface as B cells mature to become IgD+. It is not expressed by plasma cells, CD22 is found highly expressed in follicular mantle and marginal zone B-cells, and while germinal center B-cells are relatively weak. CD22 is a member of the immunoglobulin superfamily and serves as an adhesion receptor for sialic acid-bearing ligands expressed on erythrocytes and all leukocyte classes. It also associates with tyrosine kinases and play a role in signal transduction and B-cell activation.