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Home >> Antibodies >> Carcinoembryonic Antigen (CEA) / CD66

Carcinoembryonic Antigen (CEA) / CD66

Mouse Monoclonal Antibody [Clone COL-1]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
img

Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with CEA Mouse Monoclonal Antibody (COL-1).

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Western Blot analysis of human Stomach lysate using CEA Mouse Monoclonal Antibody (COL-1).

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SDS-PAGE Analysis Purified CEA Mouse Monoclonal Antibody (COL-1).

Product Details

Synonyms

Carcinoembryonic Antigen-related Cell Adhesion Molecule 5, CEACAM5, CD66, Biliary Glycoprotein (BGP-1)

Positive Control

MCF7 or 293T cells. Colon carcinoma.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Human colon carcinoma extract

Cellular Localization

Cytoplasmic and luminal surface

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2a, kappa

Mol. Weight of Antigen

80-200kDa

Specificity & Comments

This antibody recognizes proteins of 80-200kDa, identified as different members of CEA family. CEA is synthesized during development in the fetal gut and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. This MAb does not react with nonspecific cross-reacting antigen (NCA) and with human polymorphonuclear leucocytes. It shows no reaction with a variety of normal tissues and is suitable for staining of formalin/paraffin tissues. CEA is not found in benign glands, stroma, or malignant prostatic cells. Antibody to CEA is useful in detecting early foci of gastric carcinoma and in distinguishing pulmonary adenocarcinomas (60-70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+). Anti-CEA positivity is seen in adenocarcinomas from the lung, colon, stomach, esophagus, pancreas, gallbadder, urachus, salivary gland, ovary, and endocervix.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Siler K, et. al. Biotechnology Therapeutics, 1993, 4(3-4):163-81.
  2. Muraro R, et. al. Cancer Research, 1985, 45:5769-80.
  3. Robbins PF, et. al. International Journal of Cancer, 1993, 53(6):892-7.
  4. Shi ZR, et. al. Journal of Histochemistry and Cytochemistry, 1994, 42(9):1215-9.

Bioinformatics

  •  Entrez Gene ID: 1048, 634
  •  Gene Symbol: CEACAM1 & CEACAM5
  •  SwissProt: P06731
  •  Chromosome Location: 19q13.1-19q13.2
  •  Unigene: 709196
NBT00002