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Home >> Antibodies >> Beta-2 Microglobulin (Renal Failure & Tumor Marker)

Beta-2 Microglobulin (Renal Failure & Tumor Marker)

Mouse Monoclonal Antibody [Clone BBM.1]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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SDS-PAGE Analysis Purified Beta-2-Microglobulin Mouse Monoclonal Antibody (BBM.1). Confirmation of Integrity and Purity of Antibody.

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Western Blot Analysis of human THP-1 and Raji cell lysate using Beta-2-Microglobulin Mouse Monoclonal Antibody (BBM.1)

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Immunofluorescent staining of HeLa cells. Beta-2-Microglobulin Mouse Monoclonal Antibody (BBM.1) followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red)

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Flow Cytometric Analysis of PFA fixed HeLa cells using Beta-2-Microglobulin Mouse Monoclonal Antibody (BBM.1); followed by goat anti-mouse IgG-CF488 (Blue); Isotype Control (Red).

Product Details

Synonyms

B2M; Beta 2 microglobin; Beta 2 microglobulin; Beta chain of MHC class I molecules; Beta-2-microglobulin form pI 5.3; Hdcma22p

Positive Control

THP-1, Raji, HL-60 or HeLa cells. Melanomas and Lymphoma. Carcinoma of Stomach, Cervix, Endometrial, Kidney or Colon.

Known Applications & Suggested Dilutions

Flow Cyt, Western Blot, IF, IHC-Fr         More Details

Flow Cytometry (1-2ug/million cells)
Western Blot (1-2ug/ml)
Immunofluorescence (1-4ug/ml)
Immunohistochemistry (Frozen) (1-2ug/ml for 30 minutes at RT)Optimal dilution for a specific application should be determined.

Immunogen

MOLT-4 human T cell line

Cellular Localization

Cell Surface and Extracellular (Secreted)

Species Reactivity

Human. Non-Human primates.

Host / Ig Isotype

Mouse / IgG2b, kappa

Mol. Weight of Antigen

12kDa

Specificity & Comments

Recognizes a protein of 12kDa, identified as microglobulin. Major histocompatibility complex (MHC) class 1 molecules bind to antigens for presentation on the surface of cells. The proteasome is responsible for producing these antigens from the components of foreign pathogens. MHC class 1 molecules consist of an alpha heavy chain that contains three subdomains (alpha1, alpha2, alpha3) and a non-covalent associating light chain, known as beta-2-Microglobulin. Beta-2-Microglobulin associates with the alpha3 subdomain of the alpha heavy chain and forms an immunoglobulin domain-like structure that mediates proper folding and expression of MHC class 1 molecules. The alpha1 and alpha2 domains of the alpha heavy chain form the peptide antigen-binding cleft. Mutations in the beta-2-Microglobulin gene can enhance the progression of malignant melanoma phenotypes.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Brodsky F et al. 1979. Eur. J. Immunol. 9:536-45.
  2. Brodsky F et al. 1979. Immunol. Rev. 47:3-61.
  3. Parham P et al. 1983. J. Biol. Chem. 258(10):6179-86.

Bioinformatics

  •  Entrez Gene ID: 567
  •  Gene Symbol: B2M
  •  SwissProt: P61769
  •  Chromosome Location: 15q21-q22.2
  •  Unigene: 534255
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