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Home >> Antibodies >> AIF1 / Iba1 (Microglia Marker)

AIF1 / Iba1 (Microglia Marker)

Mouse Monoclonal Antibody [Clone AIF1/2493]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
img

Formalin-fixed, paraffin-embedded human Kidney stained with AIF1 / Iba1 Mouse Monoclonal Antibody (AIF1/2493).

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Formalin-fixed, paraffin-embedded human Spleen stained with AIF1 / Iba1 Mouse Monoclonal Antibody (AIF1/2493).

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SDS-PAGE Analysis Purified AIF1 / Iba1 Mouse Monoclonal Antibody (AIF1/2493). Confirmation of Integrity and Purity of Antibody.

Product Details

Synonyms

Allograft inflammatory factor 1 (AIF1); balloon angioplasty responsive transcription (BART1); IBA1; Interferon responsive transcript 1; Ionized calcium-binding adapter molecule 1; IRT1; Microglia response factor (MRF1)

Positive Control

Lymph Node, Tonsil or Kidney.

Known Applications & Suggested Dilutions

IHC-P         More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Purified recombinant human AIF1 protein (around aa 1-146) (exact sequence is proprietary)

Cellular Localization

Cytoplasmic and Cell Surface

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2c, kappa

Mol. Weight of Antigen

17kDa

Specificity & Comments

AIF1 is a cytoplasmic, calcium-binding protein that is thought to play a role in macrophage activation and function. AIF1, containing two EF domains, is induced by cytokines and Interferons. In an unstimulated state, AIF1 colocalizes with actin, and upon stimulation, translocates to lamellipodia. It is also a marker of human microglia and is expressed by macrophages in injured skeletal muscle. The gene encoding AIF1 resides in the tumor necrosis factor (TNF) cluster of genes, located in the region represented by the human major histocompatibility complex (MHC).

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Kelemen SE, Autieri MV. Expression of allograft inflammatory factor-1 in T lymphocytes. Am J Pathol2005; 167: 619-26

Bioinformatics

  •  Entrez Gene ID: 199
  •  Gene Symbol: AIF1
  •  SwissProt: P55008
  •  Chromosome Location: 6p21.33
  •  Unigene: 76364
NBT02493