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AIF1 / Iba1 (Microglia Marker) Antibody [MSVA-955M]

In Stock
Catalog Number Formulation Size Price
199-MSM19-P0
Purified Ab with BSA and Azide
20ug
$324.50
199-MSM19-P1
Purified Ab with BSA and Azide
100ug
$599.50
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
AIF1 Antibody in Numerous Tissues (IHC)

AIF1 Antibody in Numerous Tissues (IHC)

Allograft inflammatory factor 1 Mouse Monoclonal Antibody (MSVA-955M) tested on many normal and cancer tissues. The immunohistochemistry staining in these tissues aligns with the expression data in Human Protein Atlas.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1:100-1:200
IHC Protocol
Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Summary

AIF1 is a cytoplasmic, calcium-binding protein that is thought to play a role in macrophage activation and function. AIF1, containing two EF domains, is induced by cytokines and Interferons. In an unstimulated state, AIF1 colocalizes with actin, and upon stimulation, translocates to lamellipodia. It is also a marker of human microglia and is expressed by macrophages in injured skeletal muscle. The gene encoding AIF1 resides in the tumor necrosis factor (TNF) cluster of genes, located in the region represented by the human major histocompatibility complex (MHC).

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Cardiovascular, Dendritic Cell Marker
Isotype / Light Chain
IgG2c / Kappa
Cellular Localization
Cell projection, Cytoplasm, Cytoskeleton, Phagocytic cup, Ruffle membrane
Gene Name
AIF1
Positive Control
Colon: An at least moderate to strong cytoplasmic AIF1 immunostaining should be seen in mucosal macrophages.
Immunogen
Purified recombinant human AIF1 protein (around aa 1-146) (exact sequence is proprietary)
Alternate Names
Allograft inflammatory factor 1, Ionized calcium-binding adapter molecule 1, Protein G1, Allograft inflammatory factor 1 (AIF1); balloon angioplasty responsive transcription (BART1); IBA1; Interferon responsive transcript 1; Ionized calcium-binding adapter molecule 1; IRT1; Microglia response factor (MRF1)

Database Links

Entrez Gene ID
199
SwissProt
P55008

Additional Information

Clone
MSVA-955M
Chromosome Location
6p21.33
Mol. Weight of Antigen
17kDa

Related Antibodies

View All AIF1 Antibodies

Functions

  • Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.

Key References

  • Kelemen SE, Autieri MV. Expression of allograft inflammatory factor-1 in T lymphocytes. Am J Pathol2005; 167: 619-26

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

Ab produced in CHO cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
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