Contact Us

AIF1 / Iba1 (Microglia Marker) Antibody [MSVA-955M]

Additional information

Catalog Number: 199-MSM19
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

download data sheet
In Stock
Catalog Number Formulation Size Price
199-MSM19-P0
Purified Ab with BSA and Azide
20ug
$324.50
199-MSM19-P1
Purified Ab with BSA and Azide
100ug
$599.50
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
AIF1 Antibody in Numerous Tissues (IHC)

AIF1 Antibody in Numerous Tissues (IHC)

Allograft inflammatory factor 1 Mouse Monoclonal Antibody (MSVA-955M) tested on many normal and cancer tissues. The immunohistochemistry staining in these tissues aligns with the expression data in Human Protein Atlas.

  • IHC
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1:100-1:200 IHC Protocol Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Clone
MSVA-955M
Isotype
IgG2c
Light Chain
Kappa
Cellular Localization
Cell projection, Cytoplasm, Cytoskeleton, Phagocytic cup, Ruffle membrane
Gene Name
AIF1
Positive Control
Colon: An at least moderate to strong cytoplasmic AIF1 immunostaining should be seen in mucosal macrophages.
Immunogen
Purified recombinant human AIF1 protein (around aa 1-146) (exact sequence is proprietary)
Expression System
CHO
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
Ab produced in CHO cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

Related Antibodies

Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with KSP-Cadherin Recombinant Mouse Monoclonal Antibody (rCDH16/1071).
7
  • Flow
  • IHC
  • WB

Recombinant Ksp-Cadherin (Kidney-Specific Cadherin) / CDH16 Antibody

Additional information

Catalog Number: 1014-MSM4
Reactivity:

, , , ,

Gene:

Host:

Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Occludin Mouse Monoclonal Antibody (OCLN/2181).
4
  • IHC
  • WB

Occludin (Marker of Early Blood Brain Barrier Damage) Antibody

Additional information

Catalog Number: 100506658-MSM1
Reactivity:

Gene:

Host:

SDS-PAGE Analysis of Purified N-Cadherin Mouse Monoclonal Antibody (13A9). Confirmation of Integrity and Purity of Antibody.
1
  • Flow
  • IF
  • WB

N-Cadherin / Cadherin-2 / CD325 (NCAD) Antibody

Additional information

Catalog Number: 1000-MSM1
Reactivity:

,

Gene:

Host:

Formalin-fixed, paraffin-embedded human kidney stained with MICA Mouse Recombinant Monoclonal Antibody (MICA/4442). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.
5
  • IHC
  • WB

MICA Antibody

Additional information

Catalog Number: 100507436-MSM2
Reactivity:

, , , ,

Gene:

Host:

Flow cytometric analysis of PFA-fixed HeLa cells. MEF2B Mouse Monoclonal Antibody (PCRP-MEF2B-2F9) followed by goat anti-mouse IgG-CF488 (blue), unstained cells (red).
2
  • Flow
  • IF
  • WB

MEF2B (Transcriptional Activator) Antibody

Additional information

Catalog Number: 100271849-MSM1
Reactivity:

Gene:

Host:

Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with KSP-Cadherin Mouse Monoclonal Antibody (SPM594).
2
  • IHC
  • WB

Ksp-Cadherin (Kidney-Specific Cadherin) / CDH16 Antibody

Additional information

Catalog Number: 1014-MSM1X
Reactivity:

, , , ,

Gene:

Host:

Formalin-fixed, paraffin-embedded human Colon stained with Cadherin 17 / CDH17 Mouse Monoclonal Antibody (CDH17/2617).
3
  • IHC
  • WB

Cadherin 17 / LI Cadherin (Gastric Adenocarcinoma Marker) Antibody

Additional information

Catalog Number: 1015-MSM7
Reactivity:

Gene:

Host:

Formalin-fixed, paraffin-embedded human Colon stained with CDH17-Monospecific Mouse Monoclonal Antibody (CDH17/2615).
3
  • IHC
  • WB

Cadherin 17 / LI Cadherin (Gastric Adenocarcinoma Marker) Antibody

Additional information

Catalog Number: 1015-MSM5
Reactivity:

Gene:

Host:

VIEW ALL ANTIBODIES

Other Information

Functions
  • Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.
Key References
  • Kelemen SE, Autieri MV. Expression of allograft inflammatory factor-1 in T lymphocytes. Am J Pathol2005; 167: 619-26
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
199
SwissProt
P55008
Chromosome Location
6p21.33
Mol. Weight of Antigen
17kDa
Alternate Names
Allograft inflammatory factor 1, Ionized calcium-binding adapter molecule 1, Protein G1, Allograft inflammatory factor 1 (AIF1); balloon angioplasty responsive transcription (BART1); IBA1; Interferon responsive transcript 1; Ionized calcium-binding adapter molecule 1; IRT1; Microglia response factor (MRF1)

Summary

AIF1 is a cytoplasmic, calcium-binding protein that is thought to play a role in macrophage activation and function. AIF1, containing two EF domains, is induced by cytokines and Interferons. In an unstimulated state, AIF1 colocalizes with actin, and upon stimulation, translocates to lamellipodia. It is also a marker of human microglia and is expressed by macrophages in injured skeletal muscle. The gene encoding AIF1 resides in the tumor necrosis factor (TNF) cluster of genes, located in the region represented by the human major histocompatibility complex (MHC).

Worry-Free Guarantee

Should a NeoBiotechnologies antibody not meet the experimental expectations outlined on our website or datasheet, please contact our Technical Support. We unconditionally guarantee our product.

Learn More

Need Technical Help?

Our team of scientists are ready to help you with expert recommendations or technical assistance related to applications or product use.

CONTACT US

Follow Us on LinkedIn

NeoBiotechnologies develops and manufactures highly specific, validated monoclonal antibodies for cancer research and diagnostics.

Phone

Whatsapp

Quick Links

Information

© 2026 NeoBiotechnologies.  All Rights Reserved.

Terms and Conditions Privacy Policy
Contact Us