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Recombinant Tubulin beta 3 / TUBB3 (Neuronal & Stem Cell Marker) Antibody [TUBB3/7090R]

In Stock
Catalog Number Formulation Size Price
10381-RBM4-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
10381-RBM4-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
10381-RBM4-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant Tubulin beta 3 / TUBB3 (Neuronal & Stem Cell Marker) Antibody

SDS-PAGE Analysis Purified TUBB3 Recombinant Rabbit Monoclonal Antibody (TUBB3/7090R). Confirmation of Purity and Integrity of Antibody.

Recombinant Tubulin beta 3 / TUBB3 (Neuronal & Stem Cell Marker) Antibody - Image 2

Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with Tubulin beta 3 Recombinant Rabbit Monoclonal Antibody (TUBB3/7090R).

Tubulin beta 3 Antibody in Western Blot (WB)

Tubulin beta 3 Antibody in Western Blot (WB)

Western Blot Analysis of PC3 lysate using Tubulin beta 3 Recombinant Rabbit Monoclonal Antibody (TUBB3/7090R).

Tubulin beta 3 Antibody in Western Blot (WB)

Tubulin beta 3 Antibody in Western Blot (WB)

Western Blot Analysis of Human Brain tissue lysate using Tubulin beta 3 Recombinant Rabbit Monoclonal Antibody (TUBB3/7090R).

Tubulin beta 3 Antibody Validation in Western Blot on Knockdown(KD) cells.

Tubulin beta 3 Antibody Validation in Western Blot on Knockdown(KD) cells.

Western Blot validation of anti-Tubulin beta 3 antibody. Tubulin beta 3 protein expression was compared in wild-type (WT) and Tubulin beta 3 shRNA Knockdown (KD) HeLa cells (30ug of total protein). Loading control is shown below.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3.In adults, tubulin beta 3 (TUBB3) is primarily expressed in neurons and is commonly used as a neuronal marker. It plays an important role in neuronal cell proliferation and differentiation.

Product Properties & Targets

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot, Knockdown
Species Reactivity
Human
Research Areas
Cardiovascular, Developmental Biology, Immunology, Neuroscience, Infectious Disease, Signal Transduction
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cell projection, Cytoplasm, Cytoskeleton, Filopodium, Growth cone, Lamellipodium
Gene Name
TUBB3
Positive Control
Human brain or testis. PC3
Immunogen
A synthetic peptide (aa 437-450) of human Tubulin beta 3 protein (TUBB3); coupled to KLH
Alternate Names
Tubulin beta-3 chain, Tubulin beta-4 chain, Tubulin beta-III, beta 3 tubulin; CDCBM; CDCBM1; CFEOM3; CFEOM3A; FEOM3; M(beta)3; M(beta)6; MC1R; Neuron specific beta III Tubulin; Neuron-specific class III beta-tubulin; TUBB3; TUBB4; Tubulin beta-3; Tubulin beta-4; Tubulin beta-III

Database Links

Entrez Gene ID
10381
SwissProt
Q13509

Additional Information

Clone
TUBB3/7090R
Chromosome Location
16q24.3
Mol. Weight of Antigen
55kDa

Related Antibodies

View All TUBB3 Antibodies

Functions

  • Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB3 plays a critical role in proper axon guidance and maintenance. Binding of NTN1/Netrin-1 to its receptor UNC5C might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased microtubule dynamics and axon repulsion (PubMed:28483977). Plays a role in dorsal root ganglion axon projection towards the spinal cord (PubMed:28483977).

Key References

  • Osborn M et. al. European Journal of Cell Biology. 1984; 34:137-143.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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