Formalin-fixed, paraffin-embedded human thyroid carcinoma stained with Thyroid Peroxidase Recombinant Rabbit Monoclonal Antibody (TPO/6417R).

SDS-PAGE Analysis Thyroid Peroxidase Recombinant Rabbit Monoclonal Antibody (TPO/6417R). Confirmation of Purity and Integrity of Antibody.

Printable Datasheet

TPO (Thyroid Peroxidase) (Thyroid Marker) [TPO/6417R]

Name: TPO (Thyroid Peroxidase) (Thyroid Marker)
SKU: 7173-RBM16
Availability: InStock

Monoclonals Primary Antibodies Rabbit Monoclonals Recombinant Rabbit Monoclonals Recombinants

Summary

Thyroperoxidase (TPO) is a 933 amino acid, type I transmembrane glycoprotein that plays a key role in thyroid gland function and autoimmunity. It is present as a dimer on the apical surface of thyroid follicular cells. TPO functions in the iodination of tyrosine residues in thyroglobulin and phenoxy-ester formation between pairs of iodinated tyrosines to generate the thyroid hormones, thyroxine and triiodothyronine. Mutations in this gene are associated with several disorders of thyroid hormonogenesis, including congenital hypothyroidism, congenital goiter, and thyroid hormone organification defect IIA.Malignant thyroid tumors exhibit an anomaly in TPO resulting in lower affinity for anti-TPO. This antibody may aid in the differentiation between benign and malignant thyroid tumors.

Product Properties & Targets

Host

Rabbit

Species Reactivity

Human

Isotype

IgG

Cellular Localization

Cell surface, Membrane

Gene Name

TPO

Mol. Weight of Antigen

100kDa

Immunogen

Recombinant fragment of human TPO (around aa 685-804) (exact sequence is proprietary)

Database Links

Entrez Gene ID

7173

Unigene

467554

SwissProt

P07202

Additional Information

Clone

TPO/6417R

Human Gene Symbol

TPO

Chromosome Location

2p25.3

Known Applications & Suggested Dilutions

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 &degC followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Key References

Fayadat, L., et al. 1998. Human Thyroperoxidase is largely retained and rapidly degraded in the endoplasmic reticulum. Its N-glycans are required for folding and intracellular trafficking. Endocrinology 139: 4277-4285.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.