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Formalin-fixed, paraffin-embedded human duodenum stained with S100A16 Mouse Monoclonal Antibody (S100A16/7412). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.
Formalin-fixed, paraffin-embedded human skin stained with S100A16 Mouse Monoclonal Antibody (S100A16/7412). Inset: PBS instead of primary antibody; secondary only negative control.
Formalin-fixed, paraffin-embedded human colon carcinoma stained with S100A16 Mouse Monoclonal Antibody (S100A16/7412). Inset: PBS instead of primary antibody; secondary only negative control.
Western Blot Analysis of SK-BR-3 lysate using S100A16 Mouse Monoclonal Antibody (S100A16/7412).
SDS-PAGE Analysis Purified S100A16 Mouse Monoclonal Antibody (S100A16/7412). Confirmation of Purity and Integrity of Antibody.
| Document Name | |
|---|---|
| Datasheet | Download Here |
| Material Safety Data Sheet | Download Here |
| Applications | Tested Dilutions | Protocol | Note |
|---|---|---|---|
| Immunohistochemistry (IHC) | 1-2ug/ml | IHC Protocol | 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes |
| Western Blot (WB) | 2-4ug/ml | Western Blot Protocol |
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The S-100 protein family consists of a group of calcium-binding proteins that are exclusively expressed in vertebrates and exhibit cell and tissue-specific expression. The expression levels of its members differ in various pathological conditions. The extracellular functions of the S-100 family may include the ability to enhance neurite outgrowth, involvement in inflammation and motility of tumor cells. S-100A16 (S100 calcium binding protein A16), also known as AAG13 (aging-associated gene 13 protein), S100F or DT1P1A7, is a 103 amino acid nuclear and cytoplasmic protein that exists as a homodimer that binds one calcium ion per monomer. A member of the EF-hand superfamily, S-100A16 contains two EF-hand domains and is encoded by a gene that maps to human chromosome 1q21.3.
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