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Recombinant Renal Cell Carcinoma (Carbonic Anhydrase IX) Antibody [rCA9/9937]

In Stock
Catalog Number Formulation Size Price
768-MSM30-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$249.00
768-MSM30-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$539.00
768-MSM30-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$539.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
CA9 Antibody in Immunohistochemistry (IHC (P))

CA9 Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human stomach stained with CA9 Recombinant Mouse Monoclonal Antibody (rCA9/9937). Inset: PBS instead of primary antibody; secondary only negative control.

CA9 Antibody on Western Blot (WB)

CA9 Antibody on Western Blot (WB)

Western Blot Analysis of HeLa cell lysate using CA9 Recombinant Mouse Monoclonal Antibody (rCA9/9937).

CA9 Antibody on Western Blot (WB)

CA9 Antibody on Western Blot (WB)

Western Blot Analysis of Human Stomach tissue cell lysate using CA9 Recombinant Mouse Monoclonal Antibody (rCA9/9937).

Carbonic Anhydrase IX Antibody in SDS-PAGE

Carbonic Anhydrase IX Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified CA9 Recombinant Mouse Monoclonal Antibody (rCA9/9937). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Recognizes a glycoprotein of ~200kDa, identified as carbonic anhydrase IX (CAIX/gp200).In normal kidney, gp200 is localized along the brush border of the pars convoluta and pars recta segments of the proximal tubule, as well as focally along the luminal surface of Bowman’s capsule adjoining the outgoing proximal tubule. Reportedly, gp200 is expressed by 93% of primary and 84% of metastatic renal cell carcinomas. This MAb may be useful in the investigations of carcinomas of proximal nephrogenic differentiation especially those showing tubular differentiation.

Product Properties & Targets

Antibody Type
Mouse Monoclonals, Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Angiogenesis
Isotype / Light Chain
IgG2b / Kappa
Cellular Localization
Cell membrane, Cell projection, Microvillus membrane, Nucleolus, Nucleus
Gene Name
CA9
Positive Control
HeLa cells. Normal kidney, Stomach or renal cell carcinoma (IHC).
Immunogen
Extracellular N-terminal proteoglycan-like region. Epitope is between aa80-100.
Alternate Names
Carbonic anhydrase 9, Carbonate dehydratase IX, Carbonic anhydrase IX, Membrane antigen MN, P54/58N, Renal cell carcinoma-associated antigen G250, pMW1, CA9, CAIX, Carbonate dehydratase IX, Carbonic anhydrase 9, Carbonic anhydrase IX, G250, Membrane antigen MN,P54/58N, pMW1, RCC-associated antigen G250, Renal cell carcinoma-associated antigen G250

Database Links

Entrez Gene ID
768
SwissProt
Q16790

Additional Information

Clone
rCA9/9937
Chromosome Location
9p13.3
Mol. Weight of Antigen
55kDa

Related Antibodies

View All CA9 Antibodies

Functions

  • Catalyzes the interconversion between carbon dioxide and water and the dissociated ions of carbonic acid (i.e. bicarbonate and hydrogen ions).

Key References

  • Yoshida SO, et. al.Cancer Research, 1989, 49(7):1802-9.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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