Reelin [RELN/4498]

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Summary

Reelin (or Reln) is a large glycoprotein that is secreted by Cajal-Retzius cells in the forebrain and by granule neurons in the cerebellum. Reelin was shown to be mutated in reeler mice, a mutation that is associated with widespread disruption of laminated regions of the brain, leading to impaired motor coordination, tremors and ataxia. Reelin protein expression is complex and changes throughout development. Reelin appears to function upstream of Dab1, in a signaling pathway that controls cell positioning in the developing brain, and is also thought to be a direct effector of the neurotrophin BDNF.

Product Properties & Targets

Host
Species Reactivity
Isotype
Cellular Localization
Extracellular matrix, Extracellular space, Secreted
Gene Name
Positive Control
Human or mouse brain or CNS.
Immunogen
Recombinant fragment (around aa 1796-2115) of human RELN protein
Alternate Names
Reelin, LIS2; PRO1598; Reeler; Reelin; RELN; RELN_HUMAN; RL

Database Links

Entrez Gene ID
Unigene
SwissProt

Additional Information

Clone
RELN/4498
Human Gene Symbol
RELN
Chromosome Location
7q22.1
Mol. Weight of Antigen
387.5kDa

Functions

  • Extracellular matrix serine protease that plays a role in layering of neurons in the cerebral cortex and cerebellum. Regulates microtubule function in neurons and neuronal migration. Affects migration of sympathetic preganglionic neurons in the spinal cord, where it seems to act as a barrier to neuronal migration. Enzymatic activity is important for the modulation of cell adhesion. Binding to the extracellular domains of lipoprotein receptors VLDLR and LRP8/APOER2 induces tyrosine phosphorylation of DAB1 and modulation of TAU phosphorylation (By similarity).

Known Applications & Suggested Dilutions

  • ELISA (For coating, order antibody without BSA)
  • ,Immunoprecipitation (1-2ug per 100-500ug of total protein (1ml of cell lysate))
  • ,Immunofluorescence (1-2ug/ml)
  • Western Blot (1-2ug/ml)
  • ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 &degC followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Key References

  • Goffinet AM, et al. The Journal of neuroscience : the official journal of the Society for Neuroscience 27.16 (2007 Apr 18): 4243-52.

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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