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Recombinant MRP3 (Multidrug Resistance-Associated Protein 3) Antibody [rDTX1]

In Stock
Catalog Number Formulation Size Price
8714-MSM5-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$249.00
8714-MSM5-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$539.00
8714-MSM5-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$539.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Multidrug Resistance-Associated Protein 3 Antibody in Immunohistochemistry (IHC (P))

Multidrug Resistance-Associated Protein 3 Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human adrenal gland stained with MRP3 Recombinant Mouse Monoclonal Antibody (rDTX1). Inset: PBS instead of primary antibody; secondary only negative control.

MRP3 Antibody in SDS-PAGE

MRP3 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified MRP3 Recombinant Mouse Monoclonal Antibody (rDTX1). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

The human multidrug resistance-associated protein (MRP) gene family contains at least 6 members designated MRP-1 to 6. MRP3 is a 190 to 200 kD integral membrane protein which is an organic anion transporter effective in transporting chemotherapeutic drugs such as MTX, etoposide and teniposide. Northern blotting of various human tissues has indicated MRP3 to be expressed in liver, colon, pancreas and at lower levels in the kidney. In liver, MRP3 is localized to the basolateral membrane of hepatocytes and is not associated with the canalicular membrane. MRP3 is also thought to play a role in bile production and secretion in the liver.

Product Properties & Targets

Antibody Type
Mouse Monoclonals, Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Metabolism
Isotype / Light Chain
IgG2b / Kappa
Cellular Localization
Basolateral cell membrane, Basal cell membrane
Gene Name
ABCC3
Positive Control
Human bowel, pancreas or adrenal gland.
Immunogen
Recombinant prokaryotic fusion protein corresponding to a region of the cytoplasmic domains between transmembrane domains 11 and 12 of the MRP3 molecule
Alternate Names
ATP-binding cassette sub-family C member 3, Canalicular multispecific organic anion transporter 2, Multi-specific organic anion transporter D, Multidrug resistance-associated protein 3, ABC31; ATP-binding cassette sub-family C member 3 (ABCC3); Canalicular multispecific organic anion transporter 2 (CMOAT2); MLP2; MOATD; Multi-specific organic anion transporter D; Multidrug resistance-associated protein 3

Database Links

Entrez Gene ID
8714
SwissProt
O15438

Additional Information

Clone
rDTX1
Chromosome Location
17q21.33
Mol. Weight of Antigen
169/137/55/32/65kDa

Related Antibodies

View All ABCC3 Antibodies

Functions

  • ATP-dependent transporter of the ATP-binding cassette (ABC) family that bind and hydrolyze ATP to enable active transport of various substrates including many drugs, toxicants and endogenous compound across cell membranes (PubMed:11581266, PubMed:15083066, PubMed:10359813). Transports glucuronide conjugates such as bilirubin diglucuronide, estradiol-17-beta-o-glucuronide and GSH conjugates such as leukotriene C4 (LTC4) (PubMed:15083066, PubMed:11581266). Transports also various bile salts (taurocholate, glycocholate, taurochenodeoxycholate-3-sulfate, taurolithocholate- 3-sulfate) (By similarity). Does not contribute substantially to bile salt physiology but provides an alternative route for the export of bile acids and glucuronides from cholestatic hepatocytes (By similarity). Can confers resistance to various anticancer drugs, methotrexate, tenoposide and etoposide, by decreasing accumulation of these drugs in cells (PubMed:11581266, PubMed:10359813).

Key References

  • Konig, J., et al. 1999. Characterization of the human multidrug resistance protein isoform MRP3 localized to the basolateral hepatocyte membrane. Hepatology 29: 1156-1163.
  • Hirohashi T, Suzuki H, Chu X Y, et al. J. Pharmacol. Exp. Ther. 292 (1): 265–270 (2000).
  • Hirohashi T, Suzuki H, Takikawa H, et al. Journal of Biological Chemistry. 275 (4): 2905–2910 (2000).
  • van den Heuvel-Eibrink M M, Sonneveld P and Pieters R. Int. J. Clin. Pharmacol. Ther. 38 (3): 94–110 (2000).
  • Konig J, Rost D, Cui Y, et al. Hepatology. 29 (4): 1156–1163 (1999).
  • Young L C, Campling B G, Voskoglou-Nomikos T, et al. Clinical Cancer Research. 5 (3): 673–680 (1999).
  • Kiuchi Y, Suzuki H, Hirohashi T, et al. FEBS Letters. 433 (1–2): 149–152 (1998).
  • Uchiumi T, Hinoshita E, Haga S, et al. Biochemical and Biophysical Research Communications. 252(1): 103–110 (1998).
  • Kool M, de Haas M, Scheffer G L, et al. Cancer Research. 57: 3537–3547 (1997).

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab produced in CHO cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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