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Recombinant Hepatitis C virus NS3 (Nonstructural protein 3) Antibody [rMMM33]

In Stock
Catalog Number Formulation Size Price
MSM3-13221-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$249.00
MSM3-13221-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$539.00
MSM3-13221-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$539.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Hepatitis C Antibody in Immunohistochemistry (IHC (P))

Hepatitis C Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human HCV-infected liver stained with Hepatitis C Recombinant Mouse Monoclonal Antibody (rMMM33). Inset: PBS instead of primary antibody; secondary only negative control.

Hepatitis C NS3 Antibody in SDS-PAGE

Hepatitis C NS3 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified Hepatitis C virus (NS3) Recombinant Mouse Monoclonal Antibody (rMMM33). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Non structural protein 3 (NS3) of hepatitis C virus (HCV) is a protein of 67 to 70 kD that contains at least three enzymatic activities; a protease, a serine protease and an RNA helicase. NS3 is produced as part of a polyprotein of about 3000 amino acids which is encoded by the viral genome. This polyprotein undergoes proteolytic processing by a combination of cellular and viral proteases, including those provided by NS3, to yield all of the mature viral proteins. The RNA helicase activity of NS3 is thought to be involved in viral replication.

Product Properties & Targets

Antibody Type
Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Neuroscience, Neuroinflammation
Isotype / Light Chain
IgG2b / Kappa
Cellular Localization
Host cell membrane, Host cytoplasm, Host endoplasmic reticulum membrane
Gene Name
NS3
Positive Control
Hepatitis C infected tissue or cells.
Immunogen
Prokaryotic recombinant protein corresponding to non-structural protein 3 of hepatitis C virus, strain 1b
Alternate Names
NS3 protease I, NS3, NS3, NS3P, Serine protease/NTPase/helicase, p70

Database Links

Entrez Gene ID
SwissProt
P27958

Additional Information

Clone
rMMM33
Mol. Weight of Antigen
70kDa

Related Antibodies

View All NS3 Antibodies

Functions

  • Displays three enzymatic activities: serine protease with a chymotrypsin-like fold, NTPase and RNA helicase (PubMed:25551442). NS3 serine protease, in association with NS4A, is responsible for the cleavages of NS3-NS4A, NS4A-NS4B, NS4B-NS5A and NS5A-NS5B (PubMed:8035505, PubMed:8189513, PubMed:8386278). The NS3/NS4A complex prevents phosphorylation of host IRF3, thus preventing the establishment of dsRNA induced antiviral state (By similarity). The NS3/NS4A complex induces host amino acid transporter component SLC3A2, thus contributing to HCV propagation (PubMed:30341327). NS3 RNA helicase binds to RNA and unwinds both dsDNA and dsRNA in the 3' to 5' direction, and likely resolves RNA complicated stable secondary structures in the template strand (Probable). Binds a single ATP and catalyzes the unzipping of a single base pair of dsRNA (PubMed:21940894). Inhibits host antiviral proteins TBK1 and IRF3 thereby preventing the establishment of an antiviral state (By similarity). Cleaves host MAVS/CARDIF thereby preventing the establishment of an antiviral state (PubMed:16177806, PubMed:16301520). Cleaves host TICAM1/TRIF, thereby disrupting TLR3 signaling and preventing the establishment of an antiviral state (PubMed:15710891).

Key References

  • Kyono K, Miyashiro M, Taguchi I. Journal of Biochemistry (Tokyo). 135 (2): 245–252 (2004).
  • Bassendine M, Bevitt D, Burt B, et al. The 11th International Congress of Virology. Sydney, Australia. p277 (1999).
  • Lau J Y N, Krawczynski K, Negro F, et al. Journal of Hepatology. 24 (S2): 43–51 (1999).
  • Shoji I, Suzuki, Sato M, et al. Virology. 254 (2): 315–323 (1999).
  • Colin C, Vergnon P, Jullien A M, et al. The EATHIS Eco Research Group. European Acute Transfusion Hepatitis Interferon Study. Eur. J. Clin. Microbiol. Infect. Dis. 16 (3): 220–227 (1997).
  • Koch J O and Bartenschlager R. Virology. 237 (1): 78–88 (1997).
  • Belli L S, Silini E, Alberti A, et al. Liver Transpl. Surg. 2 (3): 200–205 (1996).
  • Hwang L H, Yang P M, Lai M Y, et al. Journal of Infectious Disease. 174 (1): 173–176 (1996).
  • Tai C L, Chi W K, Chen D S, et al. Journal of Virology. 70 (12): 8477–8484 (1996).
  • González-Paralta R P, Fang J W S, Davis G L, et al. Journal of Hepatology. 20: 143–147 (1994).

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab produced in CHO cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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