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Recombinant CD62E / Selectin E (SELE) / ELAM-1 Antibody [r16G4]

In Stock
Catalog Number Formulation Size Price
6401-MSM3-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$249.00
6401-MSM3-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$539.00
6401-MSM3-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$539.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
E-Selectin Antibody in Immunohistochemistry (IHC (P))

E-Selectin Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human tonsil stained with E-Selectin / CD62E Recombinant Mouse Monoclonal Antibody (r16G4). Inset: PBS instead of primary antibody; secondary only negative control.

E-Selectin Antibody in SDS-PAGE

E-Selectin Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified E-Selectin / CD62E Recombinant Mouse Monoclonal Antibody (r16G4). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

CD62E is also known as endothelial leukocyte adhesion molecule-1 (ELAM-1) or E-selectin. It is a type I membrane protein that belongs to the selectin family of surface molecules (along with CD62L and CD62P). CD62E is expressed on cytokine induced endothelial cells and mediates their binding to leukocytes. Like other selectins, CD62E is an adhesion molecule that contributes to the initial tethering and rolling of leukocytes on endothelial surfaces, a prerequisite for leukocyte extravasation into tissues. The main ligands recognized by CD62E are oligosaccharides related to sialyl-lewis X.

Product Properties & Targets

Antibody Type
Mouse Monoclonals, Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Immunology, Infectious Disease
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cell membrane
Gene Name
SELE
Positive Control
Human bone marrow, lymph node, inflamed tonsil, heart or liver.
Immunogen
Prokaryotic recombinant fusion protein corresponding to the cysteine-rich complement control protein domains of the CD62E molecule
Alternate Names
E-selectin, CD62 antigen-like family member E; Endothelial leukocyte adhesion molecule 1 (ELAM-1); Leukocyte-endothelial cell adhesion molecule 2 (LECAM2), CD62E; ELAM1; SELE; E-selectin; ELAM-1; LECAM2

Database Links

Entrez Gene ID
6401
SwissProt
P16581

Additional Information

Clone
r16G4
Chromosome Location
1q24.2
Mol. Weight of Antigen
115kDa
Expression System
CHO Cells

Related Antibodies

View All SELE Antibodies

Functions

  • Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1. May have a role in capillary morphogenesis.

Key References

  • Luo, J et al. “Noninflammatory expression of E-selectin is regulated by cell growth.” Blood vol. 93,11 (1999): 3785-91.
  • Bhatti, M et al. “Visualising E-selectin in the detection and evaluation of inflammatory bowel disease.” Gut vol. 43,1 (1998): 40-7. doi:10.1136/gut.43.1.40
  • Homeister, J W et al. “Overlapping functions of E- and P-selectin in neutrophil recruitment during acute inflammation.” Blood vol. 92,7 (1998): 2345-52.
  • Araki, M et al. “The role of E-selectin for neutrophil activation and tumor metastasis in vivo.” Leukemia vol. 11 Suppl 3 (1997): 209-12.
  • Matsuura, N et al. “Increased concentration of soluble E-selectin in the sera of breast cancer patients.” Anticancer research vol. 17,2B (1997): 1367-72.
  • Tedder, T F et al. “The selectins: vascular adhesion molecules.” FASEB journal : official publication of the Federation of American Societies for Experimental Biology vol. 9,10 (1995): 866-73.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab produced in CHO cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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