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Recombinant CD62E / Selectin E (SELE) / ELAM-1 Antibody [r16G4]

Additional information

Catalog Number: 6401-MSM3
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
6401-MSM3-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
6401-MSM3-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
6401-MSM3-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
E-Selectin Antibody in Immunohistochemistry (IHC (P))

E-Selectin Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human tonsil stained with E-Selectin / CD62E Recombinant Mouse Monoclonal Antibody (r16G4). Inset: PBS instead of primary antibody; secondary only negative control.

E-Selectin Antibody in SDS-PAGE

E-Selectin Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified E-Selectin / CD62E Recombinant Mouse Monoclonal Antibody (r16G4). Confirmation of Purity and Integrity of Antibody.

  • IHC
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Product Properties

Antibody Type
Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Clone
r16G4
Isotype
IgG1
Light Chain
Kappa
Cellular Localization
Cell membrane
Gene Name
SELE
Positive Control
Human bone marrow, lymph node, inflamed tonsil, heart or liver.
Immunogen
Prokaryotic recombinant fusion protein corresponding to the cysteine-rich complement control protein domains of the CD62E molecule
Expression System
CHO
Purification
Protein G
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1. May have a role in capillary morphogenesis.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
6401
SwissProt
P16581
Chromosome Location
1q24.2
Mol. Weight of Antigen
66.65kDa
Alternate Names
E-selectin, CD62E|ELAM|ELAM1|ESEL|LECAM2|selectin-e

Summary

CD62E is also known as endothelial leukocyte adhesion molecule-1 (ELAM-1) or E-selectin. It is a type I membrane protein that belongs to the selectin family of surface molecules (along with CD62L and CD62P). CD62E is expressed on cytokine induced endothelial cells and mediates their binding to leukocytes. Like other selectins, CD62E is an adhesion molecule that contributes to the initial tethering and rolling of leukocytes on endothelial surfaces, a prerequisite for leukocyte extravasation into tissues. The main ligands recognized by CD62E are oligosaccharides related to sialyl-lewis X.

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