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Recombinant PAX5 / BSAP (Early B-Cell Marker) Antibody [PAX5/3977R]

In Stock
Catalog Number Formulation Size Price
5079-RBM7-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
5079-RBM7-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
5079-RBM7-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

The specificity of this monoclonal antibody to its intended target was validated by HuProtTM Array, containing more than 21,000, full-length human proteins. The PAX5 gene is a member of thepaired box (PAX)family of transcription factors. The central feature of this gene family is a novel, highly conservedDNA-binding domain, known as thepaired box. The PAX proteins are important regulators in early development, and alterations in the expression of their genes are thought to contribute toneoplastic transformation. The PAX5 gene encodes theB-celllineage specific activator protein (BSAP) that is expressed at early, but not late stages of B-cell differentiation. Its expression has also been detected in developing CNS and testis; therefore, PAX5 gene product may not only play an important role in B-cell differentiation, but also in neural development andspermatogenesis.

Product Properties & Targets

Host
Rabbit
Applications
Species Reactivity
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Nucleus
Gene Name
Positive Control
Human tonsil, Spleen or lymph node.
Immunogen
Recombinant fragment (around aa 235-382) of human PAX5 protein (exact sequence is proprietary)
Alternate Names
Paired box protein Pax-5, B-cell-specific transcription factor, B cell lineage specific activator; BSAP; EBB-1; KLP; Paired box 5

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
PAX5/3977R
Chromosome Location
9p13.2
Mol. Weight of Antigen
42kDa

Functions

  • Transcription factor that plays an essential role in commitment of lymphoid progenitors to the B-lymphocyte lineage (PubMed:10811620, PubMed:27181361). Fulfills a dual role by repressing B-lineage inappropriate genes and simultaneously activating B-lineage-specific genes (PubMed:10811620, PubMed:27181361). In turn, regulates cell adhesion and migration, induces V(H)-to-D(H)J(H) recombination, facilitates pre-B-cell receptor signaling and promotes development to the mature B-cell stage (PubMed:32612238). Repression of the cohesin-release factor WAPL causes global changes of the chromosomal architecture in pro-B cells to facilitate the generation of a diverse antibody repertoire (PubMed:32612238).
  • (Microbial infection) Plays an essential role in the maintenance of Epstein-Barr virus genome copy number within the host cell by promoting EBNA1/oriP-dependent binding and transcription (PubMed:31941781). Participates also in the inhibition of lytic EBV reactivation by modulating viral BZLF1 activity (PubMed:23678172).

Key References

  • Desouki MM,et al. PAX-5: a valuable immunohistochemical marker in the differential diagnosis of lymphoid neoplasms. Clin Med Res.2010 Jul;8(2):84-8.

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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