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Recombinant PAX5 / BSAP (Early B-Cell Marker) Antibody [rPAX5/4228]

Additional information

Catalog Number: 5079-MSM8
Reactivity:

,

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
5079-MSM8-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
5079-MSM8-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
5079-MSM8-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant PAX5 / BSAP (Early B-Cell Marker) Antibody

Formalin-fixed, paraffin-embedded human tonsil stained with PAX5 Recombinant Mouse Monoclonal Antibody (rPAX5/4228).

Recombinant PAX5 / BSAP (Early B-Cell Marker) Antibody - Image 2

Formalin-fixed, paraffin-embedded human tonsil stained with PAX5 Recombinant Mouse Monoclonal Antibody (rPAX5/4228).

Recombinant PAX5 / BSAP (Early B-Cell Marker) Antibody - Image 3

SDS-PAGE Analysis of Purified PAX5 Recombinant Mouse Monoclonal Antibody (rPAX5/4228). Confirmation of Purity and Integrity.

  • IHC
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Product Properties

Antibody Type
Mouse Monoclonals, Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Dog, Human
Clone
rPAX5/4228
Isotype
IgG2a
Light Chain
Kappa
Cellular Localization
Nucleus
Gene Name
PAX5
Positive Control
Daudi or Ramos cells. Human tonsil, Raji, Spleen or lymph node.
Immunogen
A recombinant fragment (around aa 235-382) of human PAX5 protein (exact sequence is proprietary)
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Gene:

Host:

Reactivity:

, , ,

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Other Information

Functions
  • Transcription factor that plays an essential role in commitment of lymphoid progenitors to the B-lymphocyte lineage (PubMed:10811620, PubMed:27181361). Fulfills a dual role by repressing B-lineage inappropriate genes and simultaneously activating B-lineage-specific genes (PubMed:10811620, PubMed:27181361). In turn, regulates cell adhesion and migration, induces V(H)-to-D(H)J(H) recombination, facilitates pre-B-cell receptor signaling and promotes development to the mature B-cell stage (PubMed:32612238). Repression of the cohesin-release factor WAPL causes global changes of the chromosomal architecture in pro-B cells to facilitate the generation of a diverse antibody repertoire (PubMed:32612238).
  • (Microbial infection) Plays an essential role in the maintenance of Epstein-Barr virus genome copy number within the host cell by promoting EBNA1/oriP-dependent binding and transcription (PubMed:31941781). Participates also in the inhibition of lytic EBV reactivation by modulating viral BZLF1 activity (PubMed:23678172).
Key References
  • Desouki MM,et al. PAX-5: a valuable immunohistochemical marker in the differential diagnosis of lymphoid neoplasms. Clin Med Res.2010 Jul;8(2):84-8.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
5079
SwissProt
Q02548
Chromosome Location
9p13.2
Mol. Weight of Antigen
42kDa
Alternate Names
Paired box protein Pax-5, B-cell-specific transcription factor, B cell lineage specific activator; BSAP; EBB-1; KLP; Paired box 5

Summary

The PAX5 gene is a member of the paired box (PAX) family of transcription factors. The central feature of this gene family is a novel, highly conserved DNA-binding domain, known as the paired box. The PAX proteins are important regulators in early development, and alterations in the expression of their genes are thought to contribute to neoplastic transformation. The PAX5 gene encodes the B-cell lineage specific activator protein (BSAP) that is expressed at early, but not late stages of B-cell differentiation. Its expression has also been detected in developing CNS and testis; therefore, PAX5 gene product may not only play an important role in B-cell differentiation, but also in neural development and spermatogenesis.

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