Printable Datasheet

Neurofilament (NF-L) (Neuronal Marker) Antibody [NR-4]

In Stock
Catalog Number Formulation Size Price
4747-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$249.00
4747-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$539.00
4747-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$539.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Neurofilament (NF-L) (Neuronal Marker) Antibody

Formalin-fixed, paraffin-embedded human Cerebellum stained with Neurofilament Mouse Monoclonal Antibody (NR-4).

Neurofilament (NF-L) (Neuronal Marker) Antibody - Image 2

Formalin-fixed, paraffin-embedded Rat Cerebellum stained with Neurofilament Mouse Monoclonal Antibody (NR-4).

Neurofilament (NF-L) (Neuronal Marker) Antibody - Image 3

SDS-PAGE Analysis Purified Neurofilament Mouse Monoclonal Antibody (NR-4). Confirmation of Integrity and Purity of Antibody

Neurofilament (NF-L) (Neuronal Marker) Antibody - Image 4

Flow Cytometric Analysis of HEK293 cells using Neurofilament Mouse Monoclonal Antibody (NR-4) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).

Neurofilament (NF-L) (Neuronal Marker) Antibody - Image 5

Immunofluorescence staining of HEK293 cells using Neurofilament Mouse Monoclonal Antibody (NR-4) followed by goat anti-Mouse IgG conjugated to CF488 (green). Membrane stained with Phalloidin (Red).

NEFL Antibody in SDS-PAGE

NEFL Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified Neurofilament light polypeptide Mouse Monoclonal Antibody (NR-4). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

This MAb reacts with a 68kDa protein, identified as light sub-unit of neurofilaments (NF-L). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Species Reactivity
Chicken, Cow, Human, Pig, Rat
Research Areas
Neuroscience, Neural Stem Cells, Signal Transduction
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Axon, Cell projection, Cytoplasm, Cytoskeleton
Gene Name
NEFL
Positive Control
HEK293 cells; Brain, Neuroblastoma.
Immunogen
Crude neurofilament preparation from porcine spinal cord
Alternate Names
Neurofilament light polypeptide, 68 kDa neurofilament protein, Neurofilament triplet L protein, 68kDa neurofilament protein, Light molecular weight neurofilament protein, NEFL, Neurofilament light polypeptide 68kDa, Neurofilament light polypeptide, Neurofilament protein, light chain, Neurofilament subunit NF-L, Neurofilament triplet L protein, NF-L, NF68

Database Links

Entrez Gene ID
4747
SwissProt
P07196

Additional Information

Clone
NR-4
Chromosome Location
8p21.2
Mol. Weight of Antigen
68kDa

Related Antibodies

View All NEFL Antibodies

Functions

  • Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity).

Key References

  • Debus E., Weber K., Osborn M. Monoclonal antibodies specific for glial fibrillary acidic (GFA) protein and for each of the neurofilament triplet polypeptides. Differentiation 25 (2): 193-203, (1983).
  • Angelides, K.J., et. al. 1989. J. Cell Biol. 108: 1495-1506.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Reviews

There are no reviews yet.

Be the first to review “Neurofilament (NF-L) (Neuronal Marker) Antibody”

Your email address will not be published. Required fields are marked *

PARTNERSHIP OPPORTUNITIES

NeoBiotechnologies holds Exclusive rights to 10,000 recombinant and hybridoma antibody products, available for Licensing or Collaboration.

LETS TALK
Call Us Now: 1-866-2-NEOBIO (1-866-263-6246)
NeoBiotechnologies Logo
Company Info
Antibodies
Antibody Validation
Research Areas
Resources
Newsletter Signup Updates & Sales