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Nestin (Cancer Stem Cell Marker) Antibody [NES/2911]

In Stock
Catalog Number Formulation Size Price
10763-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
10763-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
10763-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Nestin is a major intermediate filament (IF) protein of embryonic central nervous system progenitor cells. It is also a component of the dynamic IF network during muscle development, where it polymerizes with Desmin and Vimentin. Nestin co-assembles with Vimentin or -internexin and forms heterodimer coiled-coil molecules which then further assemble into 10 nml IFs. Deletion of the IF consensus rod domain in nestin alters nestin localization in CNS precursor cells and radial glial cells in vivo. Nestin is a marker for neuroepithelial stem cells, glioma cells and tumor endothelial cells during rapid growth. During axon elongation of differentiation neurons, nestin localizes to the growth cones and may play a role in growth cone guidance. In the rat adrenal gland, nestin is expressed by the zona fasciculata and the zona reticularis. Nestin is also expressed by dermatomal cells and by myoblasts during the earliest stages of myogenesis.

Product Properties & Targets

Antibody Type
Host
Mouse
Applications
Species Reactivity
Isotype / Light Chain
IgG /
Gene Name
NES
Positive Control
Breast, Cerebellum, Cervix, Colon, Endometrium, Human kidney, Lung, ovary, prostate, skin and human melanoma tissue. HeLa cell lysate. SH-SY5Y cells.
Immunogen
Recombinant human NES protein fragment around aa 698-844 (exact sequence is proprietary)
Alternate Names
Nestin, ESTM 46; FLJ21841; Intermediate filament protein; Nbla00170; nes

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
NES/2911
Chromosome Location
1q23.1
Mol. Weight of Antigen
190-200kDa

Functions

  • Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells (By similarity).

Key References

  • Zhang Y, et al. Nat Commun. 2018 Sep 6;9(1):3613
  • Yamahatsu, K., et al. (2012). International Journal of Oncology, 40, 1345-1357.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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