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Homeobox protein NANOG (Cancer Stem Cell Marker) Antibody [NANOG/9704]

Additional information

Catalog Number: 79923-MSM3
Host:

Reactivity:

Gene:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
79923-MSM3-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
79923-MSM3-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
79923-MSM3-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
NANOG Antibody in SDS-PAGE

NANOG Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified NANOG Mouse Monoclonal Antibody (NANOG/9704). Confirmation of Purity and Integrity of Antibody.

NANOG Antibody in Immunohistochemistry (IHC (P))

NANOG Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human testis stained with NANOG Mouse Monoclonal Antibody (NANOG/9704). Inset: PBS instead of primary antibody; secondary only negative control.

  • IHC
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Clone
NANOG/9704
Isotype
IgG2b
Light Chain
Lambda
Cellular Localization
Nucleus
Gene Name
NANOG
Positive Control
Human testicular carcinoma.
Immunogen
Recombinant fragment (around aa100-250) of human NANOG protein (exact sequence is proprietary)
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.
Key References
  • Iv Santaliz-Ruiz, Luis E et al. “Emerging role of nanog in tumorigenesis and cancer stem cells.” International journal of cancer vol. 135,12 (2014): 2741-8. doi:10.1002/ijc.28690
  • Gawlik-Rzemieniewska, Natalia, and Ilona Bednarek. “The role of NANOG transcriptional factor in the development of malignant phenotype of cancer cells.” Cancer biology & therapy vol. 17,1 (2016): 1-10. doi:10.1080/15384047.2015.1121348
  • Vasefifar, Parisa et al. “Nanog, as a key cancer stem cell marker in tumor progression.” Gene vol. 827 (2022): 146448. doi:10.1016/j.gene.2022.146448
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
79923
SwissProt
Q9H9S0
Chromosome Location
12p13.31
Mol. Weight of Antigen
40kDa
Alternate Names
homeobox protein NANOG, Embryonic stem cell specific homeobox protein (Nanog) antibody; ENK antibody FLJ12581 antibody; hNanog antibody; Homeobox protein NANOG antibody; Homeobox transcription factor Nanog antibody; homeobox transcription factor Nanog-delta 48 antibody NANOG antibody; Nanog homeobox antibody NANOG_HUMAN antibody, homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48

Summary

The homeobox protein Nanog is a transcription factor crucial for maintaining the pluripotency of embryonic stem cells (ESCs) by inhibiting cell differentiation factors. In humans, the NANOG gene encodes this protein. Nanog operates alongside other factors such as Oct-4 and SOX2 to define ESC identity. It is also highly expressed in cancer stem cells, suggesting a potential role as an oncogene in promoting cancer development. Elevated levels of Nanog are linked to poor prognosis in cancer patients.

Nanog shows strong and specific expression in carcinoma in situ (CIS), embryonal carcinomas, and seminomas but are absent in teratomas and yolk sac tumors. Research indicates that human embryonic stem cell-related genes, including OCT4, NANOG, STELLAR, and GDF3, are expressed in seminomas and breast carcinomas. Nanog positivity is notably associated with high-grade ovarian serous carcinoma but is not observed in benign, borderline, or low-grade serous lesions. One study highlights the cellular shuttling of Nanog and its increased stromal presence during cervical cancer progression. Furthermore, Nanog overexpression has been linked to factors such as tumor differentiation, lymph node metastasis, and tumor size, with studies suggesting its predictive value for reduced overall survival (OS) and disease-free survival (DFS) in lung cancer.

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