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Recombinant Mesothelin (Mesothelial Marker) Antibody [MSLN/8391R]

In Stock
Catalog Number Formulation Size Price
10232-RBM14-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
10232-RBM14-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
10232-RBM14-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
SDS-PAGE Analysis of Purified Mesothelin Recombinant Rabbit Monoclonal Ab (MSLN/8391R). Confirmation of Integrity and Purity of the Antibody.

SDS-PAGE Analysis of Purified Mesothelin Recombinant Rabbit Monoclonal Ab (MSLN/8391R). Confirmation of Integrity and Purity of the Antibody.

Formalin-fixed, paraffin-embedded human ovarian cancer stained with Mesothelin Recombinant Rabbit Monoclonal Ab (MSLN/8391R). Inset: PBS instead of primary antibody; secondary only negative control.

Formalin-fixed, paraffin-embedded human ovarian cancer stained with Mesothelin Recombinant Rabbit Monoclonal Ab (MSLN/8391R). Inset: PBS instead of primary antibody; secondary only negative control.

Mesothelin Antibody in Western Blot (WB)

Mesothelin Antibody in Western Blot (WB)

Western Blot Analysis of OVCAR-3 lysate using Mesothelin Recombinant Rabbit Monoclonal Antibody (MSLN/8391R).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Mesothelin is a 40kDa glycosyl-phosphatidylinositol-anchored glycoprotein cleaved from a 69kDa precursor protein. Mesothelin immunoreactivity is high in cancers of the ovary (serous papillary, endometrioid and undifferentiated) and pancreas, with less frequent staining seen in adenocarcinomas of the endometrium, lung and stomach/esophagus. Mesothelin is one of the most sensitive markers for mesothelioma.

Product Properties & Targets

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cell surface. Secreted.
Gene Name
MSLN
Positive Control
OVCAR-3 cells, Lung Mesothelioma.
Immunogen
Recombinant fragment (around aa 273-407) of human Mesothelin (MSLN) protein (exact sequence is proprietary)
Alternate Names
Mesothelin | Megakaryocyte-potentiating factor | Mesothelin, cleaved form, CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor, CAK1; Megakaryocyte potentiating factor; Mesothelin; MSLN; SMR; SMRP

Database Links

Entrez Gene ID
10232
SwissProt
Q13421

Additional Information

Clone
MSLN/8391R
Chromosome Location
16p13.3
Mol. Weight of Antigen
40kDa (Mature form); 69kDa (Precursor)

Related Antibodies

View All MSLN Antibodies

Functions

  • Membrane-anchored forms may play a role in cellular adhesion.
  • Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro.

Key References

  • Chang, K. and Pastan, I. 1996. Molecular cloning of Mesothelin, a differentiation antigen present on mesothelium, mesotheliomas, and ovarian cancers. Proc. Natl. Acad. Sci. USA 93: 136-140.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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